期刊文献+

重组人肿瘤坏死因子-α联合肿瘤坏死因子受体1基因转染杀伤舌癌细胞的实验研究 被引量:4

Gene transfection of recombinant human tumor necrosis factor-α with tumor nec rosis factor-receptor-1 inhibit the proliferation of tongue carcinoma Tca 8113 cells
下载PDF
导出
摘要 目的 :观察重组人肿瘤坏死因子 α(rhTNF α)对建系细胞 (T T)作用效应与机制。方法 :反转录PCR方法克隆人TNFR1基因 ,并将其稳定转染入舌癌细胞中 ,建系并鉴定 ;通过MTT法检测、DNA降解片段琼脂糖凝胶电泳及电镜观察检测rhTNF α对T T细胞的作用效果与机制。结果 :成功构建含TNFR1基因的真核表达质粒 pcDNA3 TNFR1,并建立表达TNFR1蛋白活性的舌癌细胞系T T ;较之亲本Tca 8113细胞及转染空载体的T pc细胞 ,rhTNF α对T T细胞具有更强的细胞毒作用 ,且通过介导细胞凋亡的方式发挥其作用。结论 :重组人肿瘤坏死因子 α联合肿瘤坏死因子受体 1基因转染可有效地杀伤舌癌细胞 ,为舌癌基因治疗提供理论依据。 Objective: To study the effects of tumor necro si s factor receptor 1 (TNFR1) and recombinant human tumor necrosis factor-α(rhT NF-α) on the proliferation of human tongue carcinoma Tca8113 cells. Methods: TNFR1 gene cDNA was amplified by RT-PCR and clone into pcDNA3 vector, then transfected into Tca8113 cells. TNFR 1 protein in t he gene-transfected cells was detected by Western blot, the effect of rhTNF-α on the proliferation of the cells was studied by MTT ussay, DNA fragmentation w as observed by agarose gel eletrophoresis assay and cell morphology was studied by electron microscope. Results: pcDNA3-TNFR1 was transfeted in to Tca8113 cells and the expression of TNFR 1 protein was confirmed by W estern blot. The gene transfected cells were more sensititve to the growth inhi bition of rhTNF-α than those in the parental cells and the vector transfected cells. In the TNFR 1 transfected cells, DNA fragments and apoptotic changes were observed after the cells had been treated with rhTNF-α.Conclusion: TNFR1 gene transfection may induce Tca8113 cells to be more sensit ive to rhTNF-α. The growth inhibition may be related to apoptosic induction.
出处 《实用口腔医学杂志》 CAS CSCD 北大核心 2004年第5期522-526,共5页 Journal of Practical Stomatology
关键词 肿瘤坏死因子受体 肿瘤坏死因子 舌癌细胞 细胞凋亡 Tumor necrosis factor receptor Tumor nec rosis factor Tongue neoplasms Apoptosis
  • 相关文献

参考文献7

  • 1何荣根,徐秀祺,周晓健,张秀丽,邱蔚六,张锡泽,刘嫒如,刘桢,韩玉升,胥彬,沈祖铭,韩家娴,许良中,刘亦法.人舌鳞状细胞癌Tca8113细胞系的建立及其生物学特性[J].肿瘤,1983,7(3):97-100. 被引量:44
  • 2Boone E,Vanden Berghe T,Van Loo G,et al. Structure/Function analysis of p55 tumor necrosis factor receptor and fas-associated death domain.Effect on necrosis in L929sA cells.J Biol Chem, 2000,275(48):37596
  • 3Chinnaiyan AM,O'Rourke K, Tewari M,et al. FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis. Cell, 1995, 81(4):505
  • 4Strasser A, Newtor K. FADD/MORT1,a signal transducer that can promote cell death or cell groth. Int J Biochem Biol, 1999,31(5):533
  • 5Scaffidi C, Schmitz I, Krammer PH,et al. The role of c-FLIP in modulation of CD95-induced apoptosis. J Biol Chem, 1999,274(6):1541
  • 6Tsutsumi Y,Tsunoda S,Kamada H, et al. Molecular design of hybrid tumor necrosis factor-α. Ⅱ The molecular size of polyethylene glycol-modified tumour necrosis factor-α affects its antitumour potency. Br J Cancer, 1996,74(7):1090
  • 7刘丽,田生礼,冯彪,王国志,谢宝树,冷爱军.一种高活性人新型TNF的研制[J].中华微生物学和免疫学杂志,1996,16(4):254-258. 被引量:22

二级参考文献2

共引文献64

同被引文献45

引证文献4

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部