摘要
通过荧光光谱法和圆二色谱法研究了重组内皮抑素与稀土离子和肝素的作用 .结果表明 ,稀土离子和肝素都可以与重组内皮抑素结合并引起蛋白的荧光猝灭和二级结构的改变 ,稀土离子与重组内皮抑素的结合会影响其与肝素的相互作用 。
The interaction of recombinant endostatin with lanthanides and heparin was studied by fluorescence and CD spectra. The results indicate that the binding of lanthanides, heparin to recombinant endostatin(rhES) causes quenching of its fluorescence and conformational changes of rhES.The binding of lanthanides to rhES can affect the interaction between its protein and heparin and decrease the inhibiting activity of rhES to the proliferation of human umbilical vein endothelial cell.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2004年第10期1783-1788,共6页
Chemical Journal of Chinese Universities
基金
国家自然科学基金重大项目 (批准号 :2 9890 2 80 )资助
