摘要
目的 :研究应激性溃疡中枢调节机制及其与丘脑垂体肾上腺皮质 (HPA)轴间的关系 ,并观察左金丸的预防作用。方法 :采用改良束缚浸水法制作应激性溃疡模型。免疫组化方法检测下丘脑室旁核 c fos;用原位杂交法检测下丘脑室旁核促肾上腺皮质激素释放激素 (CRH) m RNA;用放射免疫方法检测血浆皮质醇(CORT)和促肾上腺皮质激素 (ACTH) ;同时检测胃液 p H值、溃疡指数 (IU)等指标。结果 :1模型组各指标与正常组比较均有显著性差异 (P均 <0 .0 1)。 2左金丸能够显著抑制 c fos、CRH m RNA表达 ,下调 ACTH、CORT,升高胃液 p H值 ,降低 IU(P均 <0 .0 5 )。 3雷尼替丁能够显著升高胃液 p H值 ,降低 IU(P均 <0 .0 1)。结论 :左金丸通过抑制下丘脑 c fos表达和 HPA轴通路启动 ,有效防治应激性溃疡。
Objective: To investigate the regulat or y mechanism of nerve center and the relation to hypothalamicpituit ar yadrenal(HPA) pathway of stress ulcer in rats, and the preventive effect of Zuojin pill. Methods: Stress ulcer was induced in rats by water immersion restraint stress(WRS). The expression of protein product of cfos was examined by immunohist oche mical technique in hypothalamic paraventricular nucleus. In situ hybridizati on was used to detect the expression of corticotropinreleasing hormon e(CRH) mRNA. Serum adrenocorticotrophic hormone(ACTH) and cortisol were analys e d by radioimmunoassay. Gastric mucosal index of ulcer(IU) and gastric pH level were observed commonly. Results: ①All the above indexes change d significantly under WRS compared with normal control group(all P<001 ). ②Zuojin pill could significantly alleviate the expression of cfos and CRH mR NA, reduce the level of ACTH and cortisol, increase gastric fluid pH value, de creased IU(all P<005). ③Gastric fluid pH value was increased sign ifica ntly in ranitidine group, while IU was decreased significantly (both P<0 01). Conclusion: Zuojin pill can prevent stress ulcer effectively by alleviating the expression of cfos in hypothalamic paraven tricular nucleus and the activation of HPA pathway.
出处
《中国中西医结合急救杂志》
CAS
2004年第5期276-280,共5页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
国家自然科学基金资助项目 ( 3 0 2 715 67)
广东省中医药管理局基金资助项目 ( 0 2 10 2 0 74)
