摘要
在pH 5 6的乙酸 乙酸钠缓冲溶液中 ,La3 + 与甲磺酸培氟沙星形成络合物。该络合物的荧光强度是甲磺酸培氟沙星的 1 5倍 ,最佳激发波长与最佳发射波长分别为 2 76和 4 4 0nm ,线性范围为 0 0 4～ 2 0mg·L-1,测定下限为 2 8μg·L-1。该体系的抗干扰能力与稳定性较好 ,成功地用于直接测定人血清中甲磺酸培氟沙星含量 ,测定结果为 :含量 4 79mg·L-1,RSD 6 3% ,回收率 95 %。本法亦用于测定胶囊中甲磺酸培氟沙星含量 ,测定结果与紫外
The complex of pefloxacin-La 3+ was formed in the pH 5.6 HAc-NaAc buffer. Its fluorimetric intensity was 1.5 times that of the pefloxacin, and the excitation and emission peaks of the complex were at 276 and 440 nm, respectively. The new fluorescence method was developed for determining pefloxacin in serum based on the characteristics. The content of pefloxacin in serum was 4.79 mg·L -1, the RSD and recovery were 6.3% and 95% respectively. The determination range was 0.04-2.0 mg·L -1 with the detection limit of 2.8 μg·L -1. The proposed method and UV-Vis photometric method were both applied to determining the pefloxacin in the same capsules with satisfactory results.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2004年第9期1086-1088,共3页
Spectroscopy and Spectral Analysis
