摘要
目的 研究^(32)P-磷酸铬(^(32)P-胶体)在裸鼠人胰腺癌Pc-3移植瘤间质注射后的抗癌效应及其在淋巴道的运转。方法 48只荷瘤裸鼠随机分为8组,每组6只。3.7、7.4、14.8、18.5、29.6 MBq组和冷胶体(0 MBq)对照组(1～6组)给药后14 d处死;14.8 MBq组、空白对照组(7组,8组)给药后饲养时间最长达40 d,平均30 d。通过光镜、透射电镜及免疫组织化学等方法,观察给药后^(32)P-胶体在瘤体内积聚情况、14 d瘤细胞坏死率及形态学改变,对CD34蛋白质表达阳性进行微血管密度(MVD)计数;动态观察通过淋巴道转运的^(32)P-胶体对腹股沟淋巴结(GLN)的生物学效应。结果 ^(32)P-胶体注射后主要积聚在瘤体内。1～6组瘤细胞坏死率分别为20％、45％、65％、70％、95％和4％(F=4.14～105.36,P<0.01);MVD分别为38.5±4.0、28.0±2.9、17.0±2.9、8.8±1.5、5.7±2.3和65.0±5.2(t=11.9～26.1,P<0.01)。7组瘤体缩小干瘪,结痂脱落。GLN肿大,给药组迟于并小于6组和8组;移植瘤虽包膜完整,但已存在淋巴道转移,受^(32)P射线作用后瘤细胞发生变性死亡,GLN辐射损伤程度与给药剂量呈正相关,4周后组织修复正常。结论 ^(32)P-胶体瘤体内注射抗癌效应明显,能同时治疗胰腺癌及早期淋巴转移。
Objective To study the anti-tumor effects and its mechanism of focal injection of32 P-colloid on nude mice bearing pancreatic carcinoma (Pc-3)and its radioactivities in the lymph node. Methods Forty-eight tumor-bearing mice were randomly divided into 8 groups of 6 mice. Mice of group 1-6 (3.7, 7.4, 14.8, 18.5, 29.6 MBq injected group and 0 MBq as control) were sacrificed 14 d after administration. Croup 7 and 8 (14.8 MBq and blank control) were remained alive for about 40 d with an average of 30 d. The levels of 32P in the tumor, rates of necrosed tumor cells, morphological changes and microvessel density (MVD) that was counted for the positive expression of CD34 protein were studied by microspectroscopy, electron microscopy and immuno-chemistry after injection of 32P-chromic phosphate colloid. The effects of32 P-colloid on biological behavior of groin lymph node (GLN) were dynamically observed. Results 32P-colloid mainly accumulated in the tumor after intra-tumoral injection. The rate of necrosed tumor cells was 20%, 45% , 65% , 70%, 95% and 4% (F = 4.14 -105.36, P<0.01), respectively in group 1-6, and the MVD counts were 38.5 ±4.0, 28.0±2.9, 17.0±2.9, 8.8±1.5, 5.7±2.3 and65.0±5.2 (t = 11.9-26.1, P<0.01), respectively. The tumors of group 7 were reduced, shriveled, scabbed and desquamated at last. The swelling of GLNs in the 32P groups was milder and occurred later than that of the control group (6, 8), and the radiodamaging of GLN was positively correlated with the dose administered. Although the tumors had integrated membrane but lymph node metastases were detected, and the tumor cells died degeneratively after radioactivated by 32P-colloid. The tissue returned to normal after 4 weeks. Conclusion The anti-tumor effect of 32P-colloid intratumoral injection is significant, and it is a satisfying intervention approach to treat both pancreatic carcinoma and local lymph node metastasis.
出处
《中华核医学杂志》
CAS
CSCD
北大核心
2003年第6期334-336,共3页
Chinese Journal of Nuclear Medicine
基金
江苏省科委应用基础研究基金(BJ2000004)
江苏省卫生厅科研基金(H200117)
