摘要
目的 建立高效液相色谱法测定氯霉素氢化可的松滴耳液中氯霉素和氢化可的松的含量。方法 采用反相 μBondpakC18色谱柱 ;甲醇 水 ( 70∶30 )为流动相 ;检测波长为 2 4 2nm ,外标法定量。结果 氯霉素和氢化可的松的线性范围分别为 5～30 μg/mL(r =0 .9995 ) ,1～ 6 μg/mL(r=0 .9997) ;平均加样回收率分别为 :10 0 .2 % (RSD =0 .75 % ) ,99.5 3% (RSD =0 .97% ,n =5 ) ;日内RSD分别为 0 .6 7%和 0 .6 6 % (n =5 ) ,日间RSD分别为 0 .74 %和 1.0 7% (n =4 )。结论 该方法简便、准确 ,适合该制剂质量检验分析。
OBJECTIVE To establish HPLC method for determination of chloramphenicol and hydrocortisone in ear drops. METHOD μBondpak C 18 colum was used as the stationary phase and methanol water(70∶30) as mobile phase, the detective wavelength was 242 nm, the contents were calculated by external standard method.RESULTS The linear ranges of chloramphenicol and hydrocortisone were 5 ～ 30 μg/mL( r = 0.9995)and 1 ～ 6 μg/mL( r = 0.9997), respectively; the average recoverys were 100 .2 %( RSD = 0.75 %)and 99.53%( RSD = 0.97%, n = 5); the RSD of intra day were 0.67 % and 0.66 %( n = 5); the RSD of inter day were 0.74 % and 1.07 %( n = 4).CONCLUSION This method is simple and accurate, which can be used for the quality control of this preparation.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2003年第6期507-508,共2页
Chinese Journal of Modern Applied Pharmacy
