摘要
从现在推广的小麦优良品种和有苗头的新品系中选用10个小麦基因型品种进行组织培养,从愈伤组织诱导率、绿苗分化率等方面比较了幼穗培养、花药培养、幼胚培养三种培养方式的培养效果。结果表明,幼胚培养效果最好,基因型间差异小,都能获得足够数量的再生植株。幼穗的培养效果最差,愈伤组织分化生根和绿芽十分容易,但分化成完整植株则较为困难。花药培养在基因型间差异非常明显而且有较多白化苗。此外,本研究还分析了影响小麦再生能力的因素,建立了一套高效、可靠的小麦组培再生系统,为小麦的转基因技术提供优良的受体材料。
Ten wheat genotype varieties from currently extensive cultivars which have been stable in agronomic straits, were used to carry out young inflorescence culture,anther culture and immature embryo culture. The effects of the three cultures were compared in frequency of callus induced and frequency of plantlet differentiation, The results indicated, immature embryo culture had the best effect to obtain enough quantitative regeneration plants and only a little difference was observed among different genotypes. Young inflorescence culture had the worst effect to obtain regneration plants,roots and shoots differentiated from callus were quite easy, but full plant regenerated from callus was rather difficulty. Significant difference among genotypes was presented in anther culture and regenerated a lot of white seedling. respectively. In this experiment, the factors which affect wheat regeneration ability were studied and analyzed. a set of highly effective and reliable wheat tissue culture-regene ration system was established, which can provide excellent acceptor materials for wheat transgenic technology.
出处
《激光生物学报》
CAS
CSCD
2003年第6期406-411,共6页
Acta Laser Biology Sinica
基金
国家转基因专项(ZJY-A-07)
关键词
小麦
组织培养
植株再生
wheat (Triticums aestivum L.)
tissue culture
plant regeneration
