摘要
目的 :研究HBV宫内感染机理。方法 :应用PCR技术检测 5 9例HBsAg( +)孕妇血清、阴道分泌物、羊水、胎盘及新生儿脐血清中HBVDNA。采用免疫组化ABC染色法检测胎盘中HBsAg及HBcAg。结果 :新生儿宫内感染率为 45 8% ( 2 7/5 9) ;胎盘、羊水、阴道分泌物HBVDNA( +)者宫内感染率分别为 78 8% ( 2 6/3 3 )、78 6%( 2 2 /2 8)、80 6% ( 2 5 /3 1)均显著高于其HBVDNA( -)者。免疫组化ABC染色法检测胎盘中HBsAg、HBcAg。HB sAg及HBcAg在胎盘中分布由蜕膜细胞、滋养层细胞、绒毛间质细胞至胎儿面绒毛毛细血管内皮细胞依次递减 ,有胎盘中HBsAg及HBcAg分布于上述方向相反。当羊水、阴道分泌物HBVDNA( +)时羊膜上皮细胞中检出HBsAg及HBcAg。结论 :HBV经母血和 (或 )细胞直接蔓延方式进入胎儿血循环感染胎儿 ;
Objective To study the mechanism of intrauterine infection of Hepatitis B Virus (HBV). Methods HBV DNA in serum, amniotic fluid, secretion of vagina and placenta of 59 mothers with HBsAg and HBcAg in placenta were detected by PCR. HBsAg and HBcAg in placenta were detected by immunohistochemical chiomoscopy. Results Intrauterine infection rate of placenta, amniotic flaid and secretion of vagina HBV DNA (+) (78.8%, 78.6%, 80.6%) were significantly higher than that of HBV DNA(-). The positive rate of placenta HBsAg and HBcAg was 81.4%, 61.6%. HBsAg and HBcAg in placenta decreased gradually from maternal deciduas cell, chorionis epithelion, and chorionic interstitial cell to capillary endothelium of chorion. HBsAg and HBcAg was detected amniotic epithelium in part of placenta. It placenta increased gradually from deciduas cell to capillary endothelium in placenta. HBsAg and HBcAg were detected in amniotic epithelium. HBV DNA was detected in amniotic fluid and secretion of vagina. Conclusions Intrauterine transmission may be through mother's blood and/or cell spreading directly and virginal separately ascending infection.
出处
《实用医学杂志》
CAS
2004年第8期872-874,共3页
The Journal of Practical Medicine
关键词
HBV
宫内感染
HBSAG
孕妇
免疫组化
肝炎病毒
Hepatitis B virus Intrauterine Transmission Polymerase chain Rea action immunohistochemistory
