摘要
目的 探讨不同浓度的单核细胞趋化因子 (MCP 1)对肿瘤浸润淋巴细胞 (TIL)增殖的作用 ,以及能否增加TIL对膀胱肿瘤细胞的细胞毒性。方法 观察在不同浓度的MCP 1(0 g·L-1,1× 10 -4g·L-1,1× 10 -3 g·L-1,1× 10 -2 g·L-1)作用下 ,不同时间相对应的TIL增殖的变化。用MTT法测定不同浓度的MCP 1作用后TIL对细胞毒性的影响。结果 MCP 1(1× 10 -3 g·L-1)作用 2周后 ,可显著增高TIL增殖 (P <0 .0 1) ,加强细胞杀伤率 (16 .4 % ) (P <0 .0 1)。结论 一定浓度的MCP 1可促进TIL细胞的增殖 ,并可增强TIL对EJ细胞的细胞毒性。
Objective To study the effect of monocyte chemotactic protein-1(MCP-1) on the proliferaion of tumor infiltrating lymphocytes (TILs) in patients with transitional cell cancer of bladder and their cytoxicity to bladder tumor. Methods TIL cell proliferation was assayed in the presence of MCP-1(0 g·L -1 ,1×10 -4 g·L -1 ,1×10 -3 g·L -1 , and 1×10 -2 g·L -1 ) by cell counting. Bladder caner cell line EJ was cultured as target cell and cytotoxicity of TIL with MCP-1 of different concentration was determined by 3-(4,5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide assay. Results The proliferation of TIL induced by recombinant interleukin-2 was significantly strengthened by MCP-1 in a concentration-dependent manner and the cytoxicity was increased to 28.2% at concentration of 1×10 -3 g·L -1 compared with that of control in after days (P<0.01). Conclusion The proliferation and cytoxicity of TIL can be increased by MCP-1 of a certain concentration.
出处
《现代泌尿外科杂志》
CAS
2004年第3期137-139,共3页
Journal of Modern Urology
