摘要
目的 研究高压电场在电损伤中的作用机制。 方法 将 36只新西兰大白兔 ,分为 7组。除第 1组 12只白兔外 ,其余 6组每组 4只。使用已建立的非热电损伤模型 ,2～ 4组做肌纤维平行电场实验 ,5～ 7组做肌纤维垂直电场实验。各组分别在伤后 0、2、2 4h行解剖探查及深烧伤指数(IDBI)分型 ;1组在伤后 2h行99mTc同位素扫描与γ照相 ,伤后 0、2h行组织学和超微结构等观察。结果 1组白兔局部皮肤无明显损伤 ,小极板深部组织坏死 ,大腿内侧持续肌肉痉挛 ;平行电场损伤重于垂直电场损伤 ;电流密度大、软组织量少、和电场轴线中心部位损伤相对较大 ;99mTc检查灌注相和血池相明显改变 ,光镜下观察肌纤维水肿、坏死 ;电镜下胞膜双层脂质分子间水肿分离 ,但膜无微孔 ,核变短 ,核膜部分溶解、核内糖原颗粒增加 ,线粒体和内质网肿胀 ,肌丝溶解 ,肌丝间隙扩大及糖原颗粒减少。 结论 构成高压电场的各因素导致选择性的电场内组织细胞、亚细胞和分子水平的非热损伤 。
Objective To explore the acting mechanism of electrical field in electrical injury. Methods Thirty six New Zealand white rabbits were employed in the study and were randomly divided into 7 groups. There were 12 rabbits in group 1 and 4 in each group of other 6 groups. The animal model of non thermal electrical injury previously replicated was employed in the study. Experiment with paralleled muscular fibers in electrical field was carried out in groups 2～4, while that of vertical muscular fibers in electrical field in groups 5～7. Anatomical examination was done to determine the index of deep burn injury (IDBI) in all groups of rabbits at 0, 2 and 24 postburn hour (PBH). Histological and ultrastructural examination, γ picturing and isotope scanning with 99m Tc were done in group 1 at 2 PBH. Results There was no obvious skin injury in the white rabbits in group 1. Deep tissue necrosis was identified under the small electrode.Constant muscular spasm was observed in the inner side of the thigh.The muscles in paralleled electrical field suffered more severe injury than those in vertical one.Tissue injury was more severe in those areas with higher current density,less soft tissue,and also in the central area of the axis of the electric field.There were obvious changes in the perfusion and blood pool phases in these areas as observed with the aid of 99m Tc. Light microscopic examination revealed swelling and necrosis of muscular fibers. Under electron microscopy,it was found that there were edema and dissolution with separation of lipid molecular layers of cell membrane, shortened nucleus with partial dissolution of nuclear membrane, increased hepatin granules within nucleus, swelling of mitochondria and endoplasmic reticulum, myofilament dissolution, expanded gap between myofilament and decreased number of hepatin granules. Conclusion Non thermal tissue injury in the electrical field, in terms of cell, ultrastructural and molecular levels, was induced and aggravated by all the factors consistituting high voltage electrical field.
出处
《中华烧伤杂志》
CAS
CSCD
2004年第4期235-238,共4页
Chinese Journal of Burns
基金
卫生部科研基金资助项目 (96 2 2 70 )
广东省卫生科研基金资助项目(A 19995 47)
关键词
电场
电损伤
作用机制
超微结构
组织学
Burn, electric
High voltage electrical field
Non thermal injury
