摘要
目的 了解本地区临床标本中异质性万古霉素耐药葡萄球菌 (h VRS)分离率 ,并对其生物学特性进行观察。方法 采用琼脂筛选和菌谱分析法对 5 6株甲氧西林耐药的葡萄球菌进行检测 ,同时对分离细菌的生长特性和超微结构进行观察 ,并与同源性敏感菌株及金黄色葡萄球菌标准菌株相比较。结果 本地区h VRS检出率为 14 .3% ,其中血浆凝固酶阴性葡萄球菌的分离率 (2 3.1% )明显高于金黄色葡萄球菌 (6 .7% ) ;耐药亚群与同源性敏感菌株和标准菌株比较 ,生长速度减慢 ,在固体培养基上菌落大小不等 ,在液体培养基中呈沉淀生长 ,电镜观察可见细胞壁增厚。结论 h VRS在本地区的临床标本中有一定的分离率 ,应引起重视 ;该菌的很多生物学特性与同源性敏感菌株有所不同 ,其中细胞壁增厚是比较明显的改变 ,并与该菌的耐药性有关。
Objective To investigate the prevalence of hetero-resistance of Staphylococcus to vancomycin (h-VRS) and its biological characteristics. Methods 56 strains of methicillin resistant Staphylococcus (MRS) were screened by vancomycin agar plates and confirmed by population analysis profiles. The growing states and ultra-structure of resistant strains were examined and compared with their parent strains and standard strain of S.aureus. Results Of 56 clinica strains of MRS,8 strains were detected as h-VRS,the positive rate was 14.3%,the hetero-resistance to vancomycin in coagulase-negative Staphylococci(h-VRCNS) was more often to be found than in S.aureus(h-VRSA),the positive rates were 23.1% and 6.7% respectively. Comparing with their parent strains,h-VRS showed slower growth and diversity of colony morphology,sedimentation growth in liquid medium,and thicker cell wall. Conclusion There is significantly variation between h-VRS and their parents,the thick cell wall is the most remarkable characteristics,which is responsible for the vancomycin resistance of Staphylococcus.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2004年第7期583-586,共4页
Chinese Journal of Microbiology and Immunology
基金
安徽省"十五"攻关项目基金资助 (0 10 13 0 3 9)
