摘要
目的 测定苦参碱葡萄糖注射液中苦参碱的含量及有关物质。方法 采用高效液相色谱法 ,色谱柱为C18柱 (5 μm ,4 .6mm× 2 5 0mm) ,磷酸盐缓冲液 -甲醇 (6 5∶35 )为流动相 ,检测波长 2 10nm。结果 苦参碱的线性范围为 2 .97～ 14 .85 μg·ml-1(r=0 .9999) ,氧化苦参碱的线性范围为 12 .0 6～ 4 8.2 5 μg·ml-1(r =0 .9996 )。结论 所用方法简便、快速、准确、专属性好。
OBJECTIVE To determine matrine contents and its related substances in Matrine and Glucose injection.METHODS An HPLC method was adopted.A C 18 column was used.The mobile phase consisted of phosphate buffer solution-methanol(65∶35).The UV detective wavelength was set at 210 nm.RESULTS The calibration curve of matrine was linear within 2.97 to 14.85 μg·ml -1 (r=0.9999).The calibration curve of oxymatrine was linear within 12.06 to 48.25 μg·ml -1 (r=0.9996).CONCLUSION This method is simple,rapid,accurate.Thus,it is suitable for the determination of matrine contents and its related substances in Matrine and Glucose injection.
出处
《华西药学杂志》
CAS
CSCD
2004年第4期290-291,共2页
West China Journal of Pharmaceutical Sciences
