摘要
①目的 探讨卵巢子宫内膜异位症 (内异症 )组织中PTEN基因表达及临床意义。②方法 用半定量逆转录聚合酶链反应 (RT PCR) ,检测内异症组织和正常卵巢组织中PTEN基因外显子 1 9和 5 9mRNA的表达水平 ,同时结合病人的临床资料进行分析。③结果 内异症组织中PTENmRNA外显子 1 9、5 9相对表达水平与正常卵巢组织比较 ,差异有显著性 (t=3.12 2、2 .2 0 9,P <0 .0 1、0 .0 5 )。内异症组织中PTENmRNA表达水平与病人年龄、囊肿大小无关 (t =0 .111～ 1.4 4 7,P >0 .0 5 ) ;与临床分期有关 (t =2 .6 78、2 .2 84 ,P <0 .0 5 )。④结论PTENmRNA转录水平的异常可能是内异症恶变的早期事件。
Objective To investigate the expression of PTEN gene in endometriosis of the ovary and its clinical significance. Methods The expression of PTEN mRNA was detected by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR)in tissues of endometriosis and normal ovary. Results The difference between the relative expression level of PTEN mRNA exon 1-9 and 5-9 in tissues of endometriosis and normal ovary was statistically significant (t=3.122, 2.209,P< 0.01 ,0.05). The expression level of PTEN mRNA in endometriosis of ovary was related to staging (t=2.678,2.284,P<0.01, 0.05 ), but not to ages of the patients and sizes of cysts (t=0.111-1.447,P>0.05). Conclusion PTEN gene inactivation may represent early events in the transformation of benign endometriotic cells to malignancy.
出处
《齐鲁医学杂志》
2004年第4期294-296,共3页
Medical Journal of Qilu
