摘要
目的 :观察紫杉醇协同吉西他滨对前列腺癌细胞系PC 3的体外作用 ,并探讨其可能的作用机制。 方法 :应用光镜形态学、噻唑蓝 (MTT)法、流式细胞仪和免疫细胞化学法观察 10 -6、10 -7、10 -8mol/L浓度紫杉醇和 10 -7、10 -8、10 -9mol/L浓度吉西他滨在体外单独或协同用药对前列腺癌细胞系PC 3的作用 ,对细胞DNA含量及CyclinD1表达的影响。 结果 :10 -8mol/L以上浓度吉西他滨作用 4 8h ,可增强 10 -7mol/L以上浓度紫杉醇对前列腺癌PC 3细胞系的生长抑制 [抑制率≥ (5 0 .8± 4 .2 ) % ,P <0 .0 5 ]及诱导凋亡作用 [凋亡率≥ (2 2 .9± 2 .3) % ,P <0 .0 5 ],下调CyclinD1的表达 [表达率≤ (9.6± 1.6 ) % ],与阳性对照组CyclinD1表达率 (2 5 .5± 4 .1) %相比差异有显著性 (P<0 .0 1)。吉西他滨使紫杉醇所致的G2 /M期细胞周期阻滞比例由 (70 .3± 9.7) %变为 (38.2± 4 .2 ) % ,部分地逆转了其G2 /M期细胞周期阻滞 (P <0 .0 1)。 结论 :紫杉醇和吉西他滨可以协同增强对前列腺癌细胞系PC 3的生长抑制和诱导凋亡作用 ,显示了紫杉醇和吉西他滨协同用于治疗激素非依赖性前列腺癌的可能性 ,并部分地说明了其作用机制。
Objective: To observe the synergistic effects of paclitaxel and gemcitabine on prostate cancer cell line PC-3 in vitro. Methods: Cell morphology, MTT, flow cytometer and immunocytochemical method were used to observe the effects of 10 -6, 10 -7, 10 -8 mol/L paclitaxel and 10 -7, 10 -8, 10 -9 mol/L gemcitabine on prostate cancer cell line PC-3 by single or synergistic administration for 48 hours in vitro. Results: Gemcitabine above 10 -8 mol/L enhanced the growth suppression[suppression ratio ≥( 50.8± 4.2)%, P< 0.05] and apoptosis [apoptosis ratio≥( 22.9± 2.3)%, P< 0.05] and down-regulation of the expression of cyclin D 1[expression ratio≤( 9.6± 1.6)%, P< 0.01] induced by paclitaxel above 10 -7 mol/L in PC-3 cells. Gemcitabine changed the ratio of G 2/M cell arrest induced by paclitaxel from ( 70.3± 9.7)% to ( 38.2± 4.2)%, and reversed the G 2/M arrest partially (P< 0.01). Conclusion: Paclitaxel and gemcitabine can enhance the growth suppression and apoptosis induced by paclitaxel in a synergistic way. They show great potential in the treatment of androgen-independent carcinoma of the prostate.
出处
《中华男科学杂志》
CAS
CSCD
2004年第9期658-661,666,共5页
National Journal of Andrology
关键词
紫杉醇
吉西他滨
前列腺癌
协同作用
治疗
paclitaxel
gemcitabine
prostate carcinoma
synergistic effect
therapy
