摘要
目的 :探讨精子发生阻滞与雄激素受体 (AR)和热休克蛋白 90α(HSP90α)表达的关系。 方法 :应用免疫组化二步法 ,检查 5 7例精子发生阻滞引起的不育患者睾丸活检标本AR和HSP90α的表达 ,并以 15例正常健康人睾丸组织作为对照。 结果 :在正常健康人睾丸组织中 ,AR在精原细胞、精母细胞、圆形精子细胞、支持细胞、间质细胞和肌样细胞的胞核均有表达 ,但各类细胞表达AR的强度有差异。在精子发生阻滞的睾丸组织中 ,AR高表达主要在阻滞细胞水平的胞质 ,即在核周形成一环行特异性免疫反应阳性产物带。HSP90α在正常睾丸组织精原细胞、精母细胞、支持细胞、间质细胞和肌样细胞的胞质表达 ;在精子发生阻滞的睾丸组织中 ,HSP90α为高表达 ,正常睾丸组织和精子发生阻滞的睾丸组织 ,其表达强度存在显著性差异 (P <0 .0 5 )。 结论 :AR表达部位异常可能无法介导雄激素进入核内 ,而不能实现对基因转录或翻译的调节 ,致使生精细胞难以进入细胞周期而呈现阻滞。HSP90α的高表达可能加剧AR稳定性降低 ,进而增强了AR的遍在化反应。
Objective: To explore the association of spermatogenic arrest with the expression of AR and HSP90α. Methods: The two-step immunohistochemical method was used to examine the expression of AR and HSP90α in the testicular biopsy specimens of 57 infertile men with spermatogenic arrest, compared with those taken from 15 normal healthy men. Results: AR was expressed in the nuclei of the spermatogonia, spermatocytes and round spermatids of the normal testicular biopsy, but the intensity of the AR expression showed difference in each type of cells. Furthermore, the high expression of AR in spermatogonia and spermatids was on the margin of the cytoplasm, and perinuclei(forming a ring-like structure) were very distinctive in the spermatogenic arrest testicular biopsy specimens. HSP90α was expressed in spermatogonia, spermatocytes, Sertoli cells, Leydig cells and myoid cells in the normal testicular tissues, but highly expressed in the testicular tissues with spermatogenic arrest. The immunostaining intensity of HSP90α showed significant difference (P< 0.05) between the two kinds of testicular tissues. Conclusion: The results indicate that the abnormal expression sites of AR may make the androgen receptor unable to mediate androgen into the nucleus, which may down-regulate the gene transcription activity of spermatogenic cells. It is possible that the high expression of HSP90α may lead to a dramatic decrease in AR stability and an increase in AR abiquitirylation.
出处
《中华男科学杂志》
CAS
CSCD
2004年第9期662-666,共5页
National Journal of Andrology
