摘要
本研究Ｌ－９２９作实验细胞，利用ＭＴＴ法测定了羟磷灰石（ＨＡ）及改性羟磷灰石（包括氟羟磷灰石ＦＨＡ、含磷酸盐羟磷灰石ＣＨＡ、含磷酸盐氟羟磷灰石ＦＣＨＡ）对细胞存活的影响，并在扫描电镜下观察了细胞在各材料表面的附着。结果如下：１．与空白对照细比，ＣＨＡ和ＦＨＡ组细胞的存活数明显高于空白组，分别有显著性和高度显著性差异（Ｐ＜０．０５，Ｐ＜０．０１），揭示ＣＨＡ和ＦＨＡ有促细胞生长作用。ＨＡ和ＦＣＨＡ与空白组相比则无显著性差异。２．与ＨＡ组相比，ＦＨＡ组细胞存活数明显高于ＨＡ组，两者具有极显著性差异（Ｐ＜０．０１）。ＣＨＡ和ＦＣＨＡ与ＨＡ组相比无显著性差异。３．在ＳＥＭ下，培养一小时，在各材料表面即可见到大量细胞附着；培养四小时，则可见材料表面细胞密度加大，形态更加伸展，并伸出许多丝状伪足，呈现出良好的附着。以上结果表明：各改性羟磷灰石均有良好的细胞相容性，作为种植体，其表面性状均利于细胞的贴附生长。相比之下，ＦＨＡ具有较ＨＡ更好的生物活性。
Four kinds of hydroxyapatites (HA, FHA, CHA and FCHA) have been synthesized in our laboratory. The effects of these hydroxyapatites on L-929 cells proliferation were investigated using MTT assay, and the cells attachment on the materials were observd by SEM. Noproliferation inhibition was found in each kind of the materials. The vitals of FHA group were significant higher than that of HA group. It indicated the FHA is more biocompatible and bioactive than HA. By SEM observation, good attachments on each material were found at l hr, and were-spread morphology and spindle-shaped cells could be seen at 4hr. To sum up, all the modified hydroxyapatites have good biocompatibility. As the implants, their surface shape and properties are benificial to cells attachment and growth. FHA is more bioactive than HA.
出处
《中国口腔种植学杂志》
1996年第1期1-3,共3页
Chinese Journal of Oral Implantology
基金
北京市自然科学基金
