摘要
目的 探讨用炭末明胶微量管替代其他方法检测细菌是否产明胶酶。方法 对 31种、14 5株细菌 ,采用营养明胶法、X线胶片法、常量炭末明胶法及炭末明胶微量管法进行明胶液化试验 ,并进行对比。结果 10 3株产明胶酶的细菌 ,营养明胶法 77株阳性反应 ,X线胶片法 6 0株阳性反应 ,常量炭末明胶法及炭末明胶微量管法均有 91株阳性反应。 4 2株不产明胶酶的细菌试验结果全部阴性。明胶液化平均天数 ,营养明胶法 3.3d ,常量炭末明胶法 2 .5d ,而炭末明胶微量管法仅为 1.5d。结论 采用炭末明胶微量管进行明胶液化试验反应快 ,结果明了便于观察 ,试剂用量少 ,体积小且能室温长期保存 ,优于营养明胶法 ,X线胶片法和常量炭末明胶法。
Objective To study whether gelatinase can be produced by bacteria with the method of charcoal powder gelatin microtube method. Methods Gelatin liquefaction tests were performed on 145 strains of 31 species of bacteria with the nutrition gelatin method, x-ray film method, routine charcoal powder gelatin and charcoal powder gelatin microtube method, the results of each method were compared. Results Among 103 gelatinase-producing strains, 77 strains were positive with nutrition gelatin method, 60 strains were positive with x-ray film method, 91 strains were both positive with the routine charcoal powder gelatin method and charcoal powder gelatin microtube method respectively. Among 42 strains without producing gelatinase, all the results were negative with above four methods. The average days of gelatin liquefaction were 3.3 days with nutrition gelatin method, 2.5 days with routine charcoal powder gelatin method and 1.5 days with charcoal powder gelatin microtube method. Conclusion Gelatin liquefaction tests performed by charcoal powder gelatin microtube methods can get result rapidly and clearly, and can save reagents.
出处
《中国感染控制杂志》
CAS
2004年第4期335-337,共3页
Chinese Journal of Infection Control
关键词
细菌明胶酶
炭末明胶微量管
明胶液化试验
实验室技术和方法
bacteria gelatinase
charcoal powder gelatin microtube
gelatin liquefaction test
laboratory technique and methods
