摘要
目的 :获得适于大肠杆菌表达的人γ干扰素(hIFN -γ)基因。方法 :用大肠杆菌偏嗜性密码子替换已知hIFN -γ基因编码序列中相应的密码子,将重新设计后的编码序列分为六段进行人工化学合成,通过PCR拼接技术扩增出大肠杆菌偏嗜性hIFN -γ基因编码序列 ,并进行克隆及序列分析。结果 :经序列分析证实 ,重组pGEM-hIFN -γ质粒含有与预期结果一致的hIFN -γ基因编码序列。结论 :成功进行了大肠杆菌偏嗜性hIFN -γ基因的克隆,为hIFN
Objective:In order to obtain the DNA sequence encoding human interferon-γand fitting for exˉpression in E.coli.,the codons were all changed into favorite for bacteria.Methods:The human interferon-γgene coding sequence of DNA modified according to the known E.coli favorite codon was divided into six fragˉments and synthesized them chemically.The full length DNA was connected by serious PCR.experiments.The final product of PCR was cloned into plasmid pGEM-T and the recombinant plasmid was sequenced.Results:It was proved by sequence analysis the recombinant plasmid pGEM-hIFN-γdid contain the expected DNA sequence encoding the amino-acid sequence of human interferon-γ.Conclusion:A new coding sequence of hIFN-γgene was cloned successfully,and it was predicted for enhancing expression as done by other gene in bacteria.
出处
《天津医科大学学报》
2004年第3期380-381,共2页
Journal of Tianjin Medical University
