摘要
目的 :研究透明质酸钠 (SH)在角膜中期保存中的作用。方法 :在中期保存液 (MMK液 )中 ,加入SH制成保存液Ⅰ、Ⅱ、Ⅲ (浓度分别为 0 .0 5 %、0 .0 3%、0 .0 1% ) ,以未加SH的MMK液作对照并作组间对照。对保存后的兔角膜片行大体和超微结构观察、内皮细胞苔盼兰 -茜素红染色、琥珀酸脱氢酶 (SDH)、葡萄糖 - 6 -磷酸酶 (G - 6 -P)活性检测。结果 :加入SH的三组所保存的角膜片其透明度、超微结构均好于对照组 ,角膜内皮细胞活性以实验Ⅰ组最高 ,对照组最低 ,各组间比较有显著性差异。结论 :SH对角膜内皮细胞有明显的保护作用 ,可提高保存角膜的质量 ,延长保存时间 。
Objective:To study the action of SH in mid-term cornea preservation.Methods:SH was added into mid-term preservation solution(MMK) to make the new storage media with SH of three different concentration (0.05%,0.03%,0.01%).The contrast group was MMK medium without SH.The corneal appearance,transparence and ultrastructure were observed,and trypan blue-alizarin red staining of CEC,SDH and G-6-P enzyme staining with histochemistry were made in different storage time.Results:The transparence of cornea and ultrastructure of CEC in all the experimental groups were better than that of the contrast.The activity degree of CEC was highest in group I,lowest in the contrast,and statistically significant among all the groups.Conclusion:SH plays an important role in protecting CEC in middle storage medium,and enhances the quality and prolongs the duration of the preserved corneas.The protective effect is determined by the consistence of SH.
出处
《重庆医科大学学报》
CAS
CSCD
2004年第5期664-667,共4页
Journal of Chongqing Medical University
基金
重庆市卫生局科研基金资助课题 (1999-3 0 0 3 )
关键词
透明质酸钠
内皮细胞
角膜保存
眼库技术
Sodium hyaluronate
Corneal endothelial cells
Cornea preservation
Eyebank technique
