摘要
目的 :探讨γ干扰素 (interferon γ ,IFN γ)对兔晶状体上皮细胞增殖细胞核抗原 (proliferatingcellnuclearcntigen ,PCNA)表达的影响。方法 :采用新西兰白兔白内障囊外摘除模型 ,术中囊袋内灌注BSS为A组即对照组。B、C组为治疗组 ,分别灌注 10 3 IU/mlIFN γ和 10 4IU/mlIFN γ。术后 3个月运用免疫组化法和计算机图象分析技术检测晶状体上皮细胞PCNA表达。结果 :γ干扰素组PCNA阳性表达少 ,与对照组比较有显著性差异 (P <0 0 5 ) ,高浓度抑制作用强。结论 :IFN γ有抑制兔晶状体上皮细胞PCNA表达的作用 ,IFN
Objective:To investigate the effect of interferon-γ(IFN-γ) on the expression of proliferating cell nuclear antigen((PCNA)) in rabbit lens epithelial cells(LECs).Methods:Extracapsular lens extration models were made in rabbits,and IFN-γ was irrigated into the capsular bag during operation.The rabbits were divided into 3 groups.Group A,the control group,was irrigated only with BSS during operation.Group B and group C,the treatment groups,were irrigated with IFN-γ,of concentration 10~3IU/ml,10~4IU/ml respectively.Three months later,the expression of PCNA in LECs were identified with immunohistochemical staining and automatic image analysis technology.Results:The expression of PCNA in IFN-γ group was lower than that of control group (P<0.05).Conclusions:The experiment shows that IFN-γ can inhibit the expression of the PCNA in rabbit LECs,and the inhibition of IFN-γ on PCNA is concentration-dependent.
出处
《眼科》
CAS
2004年第5期295-297,i001,共4页
Ophthalmology in China
