摘要
以绿色荧光蛋白 (GFP)作为体内研究的分子探针 ,将肺炎链球菌毒力基因与gfp融合构建肺炎链球菌自杀性荧光报告质粒 ,利用同源重组的原理 ,使gfp整合入肺炎链球菌基因组中 ,建立体内研究肺炎链球菌基因表达的荧光报告系统 ,并用荧光激发、生物学特征和生理活性测定等实验手段进行评价 ,证实这一肺炎链球菌荧光融合表达系统可在体内外报告肺炎链球菌的毒力基因表达 ,为进一步在体内分析和鉴定肺炎链球菌毒力因子的功能奠定基础。
Streptococcus pneumoniae(S.pn) is an opportunity pathogenic bacteria,environmental factors play a key role in the pathogenicity of S.pn. It is important to study virulent gene in vivo. The S.pn suicide plasmid containing gfp reporter was constructed by fusing the genes pneumolysin and gfp,in which gfp is an excellent molecule probe in vivo. The plasmid was integrated to No.22 S.pn by homologous recombination. The recombinant S.pn was gained and evaluated in aspects of fluorescence excitation, biological character and physio-activity. The results showed it is efficient and available to report the expression of virulent genes in vivo and in vitro, which will provide a new easy method for evaluating and screening the virulent genes of S.pn in vivo.
出处
《微生物学通报》
CAS
CSCD
北大核心
2004年第5期55-59,共5页
Microbiology China
基金
国家自然科学基金资助项目 (No 3 0 1 70 0 5 0 )~~
关键词
肺炎链球菌
绿色荧光蛋白
自杀质粒
Streptococcus pneumoniae,Green fluorescent protein,Suicide plasmid
