摘要
目的通过斑蝥素对G1/S期肾小管上皮细胞(RTECs)低氧损伤后细胞ATP代谢影响的研究,探讨斑蝥素稳定肌动蛋白微丝骨架系统的机制。方法将新生猪G1/S界面RTECs分为3组:实验组(氰化钠+斑蝥素)、缺氧组(氰化钠)和空白对照组,应用反相高效液相比较缺氧后各组细胞内ATP含量的变化。结果实验组细胞内ATP含量[(14.50±0.26)mmol/g蛋白]明显高于缺氧组[(4.25±0.11)mmol/g蛋白]和空白对照组[(8.58±0.13)mmol/g蛋白](P<0.01)。结论斑蝥素稳定肌动蛋白微丝骨架系统可能与抗RTECs低氧损伤后细胞能量代谢紊乱相关。
Objectives To study the mechanism of cantharidin in protecting F-actin microfilaments from disruption by hypoxic damage by observing the effects of cantharidin on intracellular ATP metabolism in G1/S-phase renal tubule epithelial cells (RTECs). Methods G1-phase RTECs were divided into cantharidin-treated group, exposed to sodium cyanide (CN) and cantharidin, hypoxic-group with CN exposure and non-treated control group. ATP levels were measured in the 3 groups with high-performance liquid chromatography. Results The concentration of CN exposure for 1 h, ATP level in the RTECs with cantharidin treatment were significantly higher than that in both hypoxic and non-treated control groups (14.50±0.26 mmol/g protein, 4.25±0.11 mmol/g protein, 8.58±0.13 mmol/g protein, respectively, P<0.01). Conclusion Cantharidin prevents the disruption of the actin cytoskeleton in hypoxic damage by preventing abnormal intracellular ATP metabolism.
出处
《第一军医大学学报》
CSCD
北大核心
2004年第10期1192-1193,1196,共3页
Journal of First Military Medical University
关键词
肾小管上皮细胞
斑蝥素
三磷酸腺苷
肌动蛋白类
低氧
renal tubule epithelial cells
cantharidin
adenosine triphosphate
actins
hypoxia
