摘要
报道了一个制备生物组织传感器的新技术。将溶胶 凝胶与植物液汁按一定比例结合 ,使天然植物中的多酚氧化酶被固定于溶胶 凝胶中 ,将其滴涂于预先涂有一层Nafion膜的铂盘电极表面 ,构成带有Nafion 溶胶凝胶 植物液汁的复合修饰膜的生物传感器 ,用于测定神经递质多巴胺。采用循环伏安法和线性扫描伏安法考察了传感器的电化学特性 ,比较了不同植物品种及其不同部位组织的生物催化活性 ;研究了乙醇用量、水酯比、植物液汁用量等因素的影响 ;考察了Nafion膜的防干扰性能。采用差示脉冲伏安法对多巴胺进行定量分析 ,线性范围为 2× 10 -6～ 1× 10 -4mol/L ;检出限为 8.0× 10 -7mol/L。与传统的组织电极相比 ,本传感器具有一系列特点。
A new technique on the preparation of biosensor was reported. SiO2 sol-gel and plant juice were mixed by a certain proportion to make the enzyme from natural plant immobilized in the SiO2 sol-gel film. The dopamine (DA) biosensor was prepared from a platinum electrode coated with a nafion film and then the modified SiO2 sol-gel layer. Cyclic voltammetry and linear scan voltammetry were used to investigate the electrochemical characteristics of the sensor. The catalysis effect of different plant species and parts was compared. The effect of dosage of ethanol, water, tetraethyl orthosilicate (TEOS) and plant juice was studied. The linearity of DA in the range of 2 x 10(-6) similar to 1 x 10(-4) mol/L with detection limit of 8.0 x 10(-7) mol/L and the correlation coefficient of 0.999 were obtained in the detrmination by differential pulse voltammetry. No interference was found from ascorbic acid, nitrite, uric acid, epinephrine and noradrenalin in the determination of DA. Satisfactory determination results of DA in samples were obtained by this method.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2004年第10期1291-1294,共4页
Chinese Journal of Analytical Chemistry
基金
广东省自然科学基金 (No .0 1110 5
No .0 3 15 77)
广西壮族自治区自然科学基金 (No .0 3 42 0 18)资助项目
