摘要
优化了S1核酸酶突变检测体系,结果表明(1)扩增法比杂交法产生异源双链DNA更节省时间;(2)PCR产物可以直接作为突变检测的底物;(3)适当降低反应温度、适当增加反应缓冲液中的NaCl浓度、适当缩短反应时间可提高突变检测效果。
The method of S1 nuclease mutation detection is modified. The results showed: (1)It was more rapid of the method of co-amplification than hybridization to produce the heteroduplex DNA;(2)The PCR product could be used as the substrate directly;(3)The mutation detection effect was improved by lowing the reaction temperature or shortening the reaction time or improving the concentration of the NaCl in the buffer properly.
出处
《中国农学通报》
CSCD
2004年第5期21-22,32,共3页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目(No:39970526)。
