摘要
观察破骨细胞在离体状态下的骨吸收功能。新鲜牛皮质骨经锯式切片机横切成５０μｍ厚０．５×０．５ｃｍ大小骨片。参照已建立的破骨细胞分离培养方法，从新生Ｗｉｓｔａｒ大鼠四肢长骨分离破骨细胞，培养于２．５ｃｍ细胞培养皿内或上述骨片上，相差倒置显微镜或扫描电镜观察，可见培养的细胞具有典型破骨细胞的形态特点，接种子骨片上的破骨细胞分别培养１、３、５、７天，扫描电镜观察，可见破骨细胞能在骨片表面形成吸收陷窝，其形态多样，深浅不一，边界清晰，底面粗糙，而且随培养时间延长，陷窝扩大，数量增多。结论，破骨细胞在体外培养条件下具有良好的骨吸收功能，可进行药物干预的研究。
We observed the bone-resorptive function of osteoclasts in vitro. Fresh cow conical bones were cut across into slices 50μm in thickness and 0. 5×0. 5cm in area with saw-like microtome. According to the method established previously,the osteoclasts were disaggregated from the neonatal Wistar rat long bones and settled onto 2. 5cm culture dishes or the cut bone slices. Under phase-contrast-inverted microscope or scanning electron microscope,it was found that the cultured cells had typically morphological characteristics of osteoclasts. The osteoclasts settled onto bone slices were cultured for 1,3, 5 and 7 days,separately. Scanning electron microscopy showed that the osteoclasts could produce excavation on the bone surface which were of complex morphology,different depth,a well-defined outline and distinctive fibrillar base. Furthermore,with the prolonged culture,the area of the excavation enlarged and its number increased. It is concluded that the osteoclasts have good bone-resorptive function on the condition of culture in vitro and can be used to study the efficacy of drug intervention.
出处
《中国骨质疏松杂志》
CAS
CSCD
1997年第3期3-5,共3页
Chinese Journal of Osteoporosis
