期刊文献+

乙型肝炎病毒前C区A83突变检测及临床研究 被引量:3

A Detect of Hepatitis B Virus Precore A83 Mutations and Clinical Investigation
下载PDF
导出
摘要 目的 应用扩增抗拒突变系统 (AmplificationrefractorymutationssystemARMS)检测乙型肝炎病毒前C区A83突变 ,同时应用基因测序法评价其可行性 ,并探讨乙型肝炎病毒前C区A83突变与患者临床病情及血清中HBeAg表达的关系。 方法 采用ARMS对 13 9例乙型肝炎病毒感染者血清中HBVDNA前C区nt1896位点进行检测 ,并对其中 62例标本采用巢式PCR扩增HBVDNA前C区基因片段后直接测序。 结果  62例标本中 ,ARMS检测出A83突变3 5例 ,基因测序法检测出A83突变 41例 ,A83突变例数差异无显著性 ( χ2 =1.2 3 ,P >0 .0 5 ) ;在ARMS检测的 13 9例标本中 ,HBeAg阳性标本 5 7例 ,有 5例A83突变 ,占 8.8% ,HBeAg阴性的 82例标本中有 5 6例A83突变 ,占 68.3 % ( χ2 =48.4,P <0 .0 1) ;2 2例急性肝炎 (AH )患者未检测到A83位点突变 ,79例慢性肝炎患者 (CH )有 3 1例A83位点突变( 3 9.2 % ) ,2 9例肝硬化患者 (HLC)中有 2 1例A83位点突变 ( 72 .4% ) ,9例肝癌患者 (HCC)均有A83位点突变 ( 10 0 % )。 结论 HBeAg阴性患者病毒前C区A83突变率明显高于HBeAg阳性患者 ;前C区A83突变与患者感染乙型肝炎病毒后的慢性化有关 ;ARMS检测方法简单、快速、结果可靠 ,可以满足临床对乙肝病毒前C区A83突变分析的需要。 Objective Toestimatet hefeasibil ityofampl ificatio nrefra ctorymu tationss ystem (ARMS)indetecti ngthehepatitisBvi ruspre coreA83mutations ,andst udythe relation shipbetw eenA83mu tationsandpatient’scondit onorexpr essofseru mHBeAg Methods Ampli ficati onrefactorymutationssystem wereused todetectA83mutations ,nes tpolym erasech ainreactionand seque nceana lysisofhepatitisBviruspreco rege newereus edtocompa rewithARMS . Results Therew ereforty -oneandthirty -sixA8 3mut ationsinsixty -twopati entsbys equenceanalysi sorARMS ,and thenu mberofA83 mutationsw erenotsignificant( χ2 =1.2 3 ,P >0 .0 5 ) .Onehundre dand thirtyninesa mplesweredetectedbyARMS ,there weref iveA 83mutati onsamongfi fty -sevenp atientswith HB eAgpositiveandfifty -sixA83mu tationsam ongeighty -twopa tientswithHBeAgneagitive .TherewerenoA83mutationinacutehepatitispatients .AllhepatocellularcarcinomapatientshadA83mutations ,thirty -oneandtwenty -oneA83mutationsexistsinseventy -ninechronichepatitisandtwenty -nineliver cirrhosispatients . Conclusions A83m utatio nsinpatientswithserum HBeAgneagitiv ewereobviouslym orethanthatin patients withHBeAgpositive .A83mu tatio nsinthehepatitisBvirusprecoregen em aybeassoci ated withthedev elopmentofseverel iverdisease .Amplifica tion refractorymutationssysteminstud yo fHB VDNAA83mutationsissimple ,rapid ,accura teandcouldb eusedwidelyforcl inicalinvestigation .
出处 《实用预防医学》 CAS 2004年第5期896-898,共3页 Practical Preventive Medicine
基金 常州市科委基金项目
关键词 乙型肝炎病毒 序列分析 扩增抗拒突变系统 A83 Hepatitis Bvirus Sequencean alysis Amplification refractory mutations system A83
  • 相关文献

参考文献10

二级参考文献5

共引文献14055

同被引文献16

  • 1邱利玲.167例肝炎患者凝血四项指标检测结果分析[J].国际检验医学杂志,2007,28(6):569-569. 被引量:7
  • 2CARMAN W F,JACYNA M R,HANZIYANNIS,et al.Mutation preventing formation of hepatitis B e antigen in patients with chronic hepatitis B virus infection[J].Lancet,1989,2:588-590.
  • 3HUNT C M,McGILL J M,ALLEN M I,et al.Clinical relevance of hepatitis B viral mutations [J].Hepatology,2000,31:1037-1044.
  • 4LINDH M,HORAL P,DHILLON A P,et al.Hepatitis B virus carriers without precore mutations in hepatitis B e antigen-negative stage show more severe liver damage[J].Hepatology,1996,24:494-501.
  • 5BAYRAKTAR Y,KOSEOGLU T,TEMIZER A,et al.Relationship between the serum alanine aminotransferase level at the end of interferon treatment and histologic changes in wild-type and precore mutant hepatitis B virus infections[J].J Viral Hepat,1996,3:137-142.
  • 6Carman WF, Jacyna MR, Hanziyannis, et al. Mutation preventing formation of hepatitis B e antigen in patients B with chronic hepatitis B virus infection[J]. Lancet, 1989, 2 : 588 - 590.
  • 7Hunt CM, McGill JM, Allen MI. et al. Clinical Relevance of Hepatitis B Viral Mutations [J]. Hepatology, 2000, 31(5):1037- 1044.
  • 8Lindh M, Horal P, Dhillou AP, et al. Hepatitis B virus carriers without precore mutations in hepatitis B e antigen- negative stage show more severe liver damage[J]. Hepatology, 1996, 24:494 - 501.
  • 9Bayraktar Y, Koseoglu T, Temizer A, et al. Relationship between the serum alanine aminotransferase level at the end of interferon treatment and histologic changes in wild - type and precore mutant hepatitis B virus infections[J]. J Viral Hepat, 1996, 3:137- 142.
  • 10Carman WF,Jacyna MR, Hanziyannis, et al. Mutation preventing formation of hepatitis B eantigen in patients with chronic hepatitis B virus infection[J]. Laneet, 1989,2: 588 - 590

引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部