摘要
目的 研究甲醛对小鼠淋巴及睾丸组织O6-甲基鸟嘌呤 -DNA甲基转移酶 (MGMT)表达的影响。 方法选择健康雄性昆明小鼠 40只 ,随机分为 4组 :甲醛低剂量组 ( 2 .5mg/m3 )、中剂量组 ( 5mg/m3 )、高剂量组 ( 10mg/m3 )、对照组 (无甲醛暴露 ) ,每组 10只。除阴性对照组外 ,其余 3组每日吸入甲醛 1次 ,每次 4h ,连续 9周。应用链霉亲和素生物素—过氧化物酶 (SABC)免疫组化法对小鼠胸腺、脾及睾丸组织中MGMT进行检测。 结果 高剂量染毒组小鼠胸腺、脾和睾丸组织中MGMT表达降低 ,与对照组比较差异具有显著性 (P <0 .0 5或P <0 .0 1)。 结论 甲醛对小鼠淋巴和睾丸组织MGMT表达可能具有抑制作用。
Objective To study the effects of formaldehyde(FA) on expression of MGMT in mice thymus, spleen and testicular. Methods 40 male kunming mice were selected and randomly divided into 4 groups, 10 mice per group, FA low-dose group(2.5 mg/m3), FA middle-dose group(5 mg/m3), FA high-dose group(10 mg/m3), experimental control group(exposed no FA). Except for the control group, the other 3 groups were exposed to FA by inspiration in a static total enclosure chamber 4 hours once a day for 9 consecutive weeks. After exposure, the MGMT in thymus, spleen and testicular was examined by SABC immunohistochemical staining. Results The expression of MGMT in thymus, spleen and testicular in high experimental groups were significantly lower compared with the control (P<0.05 or P<0.01). Conclusion FA could decrease the expression of MGMT in thymus, spleen and testicular in mice
出处
《实用预防医学》
CAS
2004年第5期916-917,共2页
Practical Preventive Medicine
