摘要
目的 研究小鼠胸腺树突状细胞的分布与形态。方法 运用光镜、电镜和树突状细胞标志物 (CD2 0 5、CD1a与MIDC 8)免疫组化标记方法观察小鼠胸腺树突状细胞的分布、含量与形态。结果 小鼠胸腺树突状细胞在皮髓交界区分布最多 ,然后依次为皮质区和髓质区。树突状细胞约占胸腺细胞总数的 2 %。胸腺树突状细胞表面形成胞浆突起向 ,周围包绕的淋巴细胞间延伸 ,核不规则 ,多有凹陷 ,胞浆低电子密度 ,细胞器稀少且不含溶酶体。结论 多种树突状细胞标志物联合标记 ,结合光镜、电镜形态学观察 。
Objective To investigate the morphology and the distributon of thymus dendritic cells (TDCs) in mouse. Methods TDCs in mouse were observed and analyzed by the electron microscopy and the immunohistochemistry with DCs marker (including CD205, CD1a and MIDC 8). Results There were the most TDCs in the boundary area of thymus cortex and medulla, lesser TDCs in the cortex, and the least in the medulla. TDCs made up to 2 per cent of all cells in thymus. On TDCs surface there were many processes which extended into diastema surrounding lymphocytes. The nucleus was irregular in shape and there were intentations. The cytoplasm was electron lucent and there were few organelles without any lysosme. Conclusion It may effectively count TDCs and display their morphological character that TDC be jointly labeled by the diversiform markers and its ultrastructure observed.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第9期813-815,共3页
Medical Journal of Chinese People's Liberation Army
基金
全军医学科研"十五"计划面上项目资助课题 (编号 0 1MA2 1 0 )
