摘要
目的 建立同时测定人血浆中苯巴比妥 (PB)、苯妥英 (PHT)、卡马西平 (CBZ)和氢化可的松 (HC)浓度的方法。方法 采用C18柱 (15 0mm× 4 6mm ,5 μm) ,以 0 0 1mol·L-1NaH2 PO4(pH 3 0 ) -甲醇 -乙腈 (4 7∶4 9∶4 )为流动相 ,莫沙必利为内标 ,检测波长 2 4 5nm。结果 标准曲线线性范围PB为 12 5～ 2 0 0 0 μmol·L-1,PHT为 6 2 5～ 10 0 0 μmol·L-1,CBZ为 5 0～ 80 0 μmol·L-1,HC为 80～ 12 80 μg·L-1。萃取回收率PB为 77 95 %～ 87 83% ,PHT为 84 97%～ 86 4 5 % ,CBZ为 79 2 1%～ 93 36 % ,HC为82 90 %～ 93 36 % ;方法回收率PB为 97 2 8%～ 10 0 5 % ,PHT为 96 4 3%～ 10 0 4 2 % ,CBZ为 95 6 3%～ 10 0 4 2 % ,HC为 98 82 %～10 0 98% ;日内测定RSD则分别为 1 2 1%～ 5 95 %、0 6 5 %～ 5 5 2 %、3 4 %～ 9 9%、1 70 %～ 6 0 3% ;日间测定RSD则分别为1 17%～ 6 76 %、3 6 4 %～ 7 2 6 %、0 96 %～ 4 81%、1 4 4 %～ 7 4 2 %。结论 本法快速、灵敏、准确 ,可用于临床同时监测
OBJECTIVE To develop a method for the determination of the concentrations of phenobarbital(PB), phenytoine(PHT), carbamazepine(CBZ) and hydrocortisone(HC) simultaneously in human plasma by RP-HPLC. METHODS The plasma sample was extracted with 2 ml ethyl acetate,then separation was achieved on the Tsk-Gel ODS-100s(150 mm×4.6 mm,5 μm) column. The mobile phase consisted of 0.01 mol·L -1 NaH 2PO 4(pH 3.0)-CH 3OH-CH 3CN(47∶49∶4) and the effluent was measured at UV 245 nm. Ketoprofen was used as the internal standard. RESULTS The calibration curves of PB, PHT, CBZ and HC were linear in the range of 12.5-200.0 μmol·L -1, 6.25-100.0 μmol·L -1 , 5.0-80.0 μmol·L -1 and 80-1280 μg·L -1. The extraction recoveries of PB, PHT, CBZ and HC were 77.95%-87.83%, 84.97%-86.45%, 79.21%-93.36% and 82.90%-93.36%, respectively. The method recoveries of PB, PHT, CBZ and HC were 97.28%-100.5%, 96.43%-100.42%, 95.63%-100.42% and 98.82%-100.98%, respectively. The inter-day RSD were 1.17%-6.76%, 3.64%-7.26%, 0.96%-4.81% and 1.44%-7.42%, respectively. The within-day RSD were 1.21%-5.95%, 0.65%-5.52%, 3.4%-9.9% and 1.70%-6.03%, respectively. CONCLUSION The assay is simple, rapid, and sensitive. It is suitable to be used for TDM of PB, PHT, CBZ and HC in clinical.
出处
《华西药学杂志》
CAS
CSCD
2004年第5期357-359,共3页
West China Journal of Pharmaceutical Sciences
