摘要
以云南元江普通野生稻为供体亲本 ,在特青的遗传背景下构建了一套BC3 高代回交群体。利用 117个SSR标记分析 383个BC3 F2 株系的基因型 ,采用单标记分析法对控制元江普野株高和抽穗期的QTL进行分析。在北京和合肥两个地点试验结果表明 ,控制株高的QTL分布在第 1染色体上 ,在RM 10 4附近有一个QTL ,与sd 1位置相当 ,其对表现型变异的贡献率在两个地点分别为 2 7%和 2 8% ,其加性效应值分别为 2 6 2 4cm和 2 6 2 8cm,来自野生稻的等位基因显著提高回交群体的株高 ;在第 1、3、7、8、11染色体共检测到 6个控制抽穗期QTL ,其中第 8染色体RM 2 5附近控制抽穗期的QTL在两个地点的贡献率分别为 13%和 15 % ,加性效应值为 4 6 0d和 3 6 5d 。
An advanced backcross strategy was used to identify quantitative trait loci (QTLs) associated with plant height and days to heading in the BC_3F_2 population derived from an accession of common wild rice (Oryza rufipogon Griff.) from Yuanjiang in Yunnan Province of China,as the donor,and an elite Indica cultivar 'Teqing',as the recipient.Based on analyses of 116 SSR markers distributed throughout the rice genome and by (using) single-point analysis,four putative QTLs derived from O.rufipogon were detected for plant height on chromosome 1,and 6 QTLs for days to heading on chromosome 1,3,7,8 and 11.All Alleles of QTLs for plant height from O.rufipogon could increase plant height of the backcross population.One QTL near RM104 on chromosome 1,explaining 27% and 28% of phenotypic variance and additive value reached 26.24 cm and 26.28 cm respectively in Beijing and Hefei,was detected.The location of this QTL is corresponding to sd-1.One QTL near the marker RM25 on chromosome 1,explaining 13% and 15% of phenotypic variance was identified.The additive value of this QTL reached 4.60 days and 3.65 days respectively in Beijing and Hefei,and the allele from (O.rufipogon could) delay the backcross population's days to heading.
基金
国家自然科学基金资助项目 (编号 :3 0 2 70 80 3 )~~
关键词
普通野生稻
亚洲栽培稻
株高
抽穗期
QTL
高代回交群体
common wild rice
Asian cultivated rice
QTL
plant height
days to heading
advanced backcross analysis
