摘要
目的 :观察益骨胶囊含药血清对体外培养的大鼠成骨细胞 (OB)表达NO、NOS的影响。方法 :(1)将 4 0只 12月龄SD大鼠分为益骨胶囊组 (高、中、低剂量组 )和生理盐水对照组 ,制备含药血清和对照血清 ;确定最佳灌胃剂量组和最佳血清添加浓度。 (2 )分离、培养新生SD大鼠的颅骨成骨细胞 ,传至第 3代后分为 2组 (含药血清组和空白血清对照组 ) ,分别在培养 2 4h、4 8h和 72h后用RT PCR和试剂盒检测OB表达NOSmRNA的量及其活性变化和OB分泌NO的量。结果 :益骨胶囊含药血清组在 2 4h、4 8h和 72h时OB表达NOSmRNA的量及其活性和分泌NO的量均明显高于空白血清对照组 (P <0 0 1或P <0 0 5 )。结论 :益骨胶囊含药血清能促进OBNO的分泌和NOS的表达 。
Objective:The effects of Yigu Capsule on NO and NOS of osteoblasts were studied.Methods:(1)Forty 12-month old Sprague-daweley female rats were divided into randomly four groups (Yigu Capsule high dose group,medium dose group and low dose group;saline group),the drug-containing serum and control serum were prepared.(2)The new-born Sprague-daweley rat Osteoblasts were cultured with different Yigu Capsule drug-containing serum concentration and at different exposure time.The mRNA levels of eNOS and iNOS were measured by RT-PCR;the activities of eNOS and iNOS were measured by test kit at different exposure time.NO secretion of osteoblasts was observed,too.Results:The mRNA levels and the activities of eNOS and iNOS and NO secretion of osteoblasts were higher than the control groups respectively after 48,72 and 96 hours (P<0.01 or P<0.05). Conclusions: Yigu Capsule drug-containing serum can promote NOS mRNA and NOS activities expressions and NO secretion of osteoblasts.These results may be parts of the mechanism of Yigu Capsule preventing and treating osteoporosis.
出处
《中药材》
CAS
CSCD
北大核心
2004年第8期593-596,共4页
Journal of Chinese Medicinal Materials
基金
广东省自然科学基金(No990 475 )
广州市科技攻关计划重点项目( 2 0 0 2Z3 E5 0 71)
广东省中医药局科研基金(No10 3 0 42 )资助项目
