摘要
目的 探讨人蛔虫Ⅱ期幼虫提取物诱导体外培养的人肺上皮细胞A549凋亡 ,以及提取物浓度和作用时间与细胞凋亡的相互关系。 方法 根据四氮唑盐酶还原法 (MTT)结果 ,选用 5种不同浓度的提取物诱导人肺上皮细胞凋亡。分别在诱导后的 5个时间段 ,采用苏木素 伊红 (HE)染色盲法计数和二苯胺法检测DNA断裂率 ,观察肺上皮细胞凋亡情况。同时对某个时间和浓度组的样本 ,用DNA琼脂糖凝胶电泳和流式细胞仪检测细胞凋亡。 结果不同浓度人蛔虫Ⅱ期幼虫提取物诱导A549细胞凋亡 ,在 5h之内细胞凋亡率随提取物浓度增加而增加 ,两者呈正相关关系 ,且细胞凋亡率显著高于对照组 (P <0 0 5 ) ,5h细胞凋亡率达高峰 (65 2 % )。 结论 人蛔虫Ⅱ期幼虫提取物可诱导人肺上皮细胞凋亡 ,细胞凋亡与其提取物浓度呈明显的相关关系。在时间上表现为双向变化关系 。
Objective To induce the apoptosis of human alveolar epithelial cells(A\-\{549\}) by the extraction of the second stage larvae of Ascaris lumbricoides and investigate the extraction concentration and inducing time related to the apoptosis. \ Methods\ Following to the results of Microculture Tetrazolium Test (MTT), five concentrations of the extraction of the second stage larvae were chosen to induce the apoptosis of A\-\{549\} cells. Meanwhile, control groups without the inducement were set up. For each group, observation was made at five time points since the start of inducement, to assess the existence of apoptosis and percentage of cells showing characteristics of apoptosis. HE stain and diphenylamine reaction methods were used to assess the cell apoptosis. Agarose gel electrophoresis of DNA and flow cytometry were also employed to confirm the apoptosis for some groups. \{\ Results \ \}Observations indicated that the apoptosis ratio of A\-\{549\} cells induced by the extraction at different concentrations were significantly higher than that of the control cells (P<0\^05). At most time points of observation, the apoptosis ratio increased with the increase of concentration, indicating a positive correlation between them. For each concentration group, the apoptosis ratio increased as the inducing time prolonged until a peak appeared at 5 h of the inducement. \ Conclusion \ The extraction of the second stage larvae of Ascaris lumbricoides can induce apoptosis of human alveolar epithelial cells in vitro with a concentration\|dependent pattern. With regard to the relationship of apoptosis to the time of inducement, two trends were revealed and the relationship also influenced by the concentration of the larvae extraction.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2004年第4期209-212,共4页
Chinese Journal of Parasitology and Parasitic Diseases
