摘要
目的 研究雷帕霉素 (RPM)对人树突状细胞 (DC)功能的影响。方法 分离外周血单个核细胞 (PBMC) ,在含粒细胞巨噬细胞集落刺激因子 (GM CSF)、白介素 4 (IL 4 )以及有或无RPM的培养条件下制备DC。用流式细胞仪检测DC表面共刺激分子CD86 (B7 2 )的表达 ;混合淋巴细胞反应(MLR)检测DC对同种异体T淋巴细胞的刺激能力 ;ELISA法测定MLR上清液中的细胞因子。结果 与对照组比较 ,经RPM处理的DC表面CD86的表达明显降低 ;对T淋巴细胞刺激的能力下降 ;MLR中致炎细胞因子 (IL 1β ,IL 6 ,TNF a)浓度降低。RPM的这些作用呈浓度依赖性。 结论 雷帕霉素对人树突状细胞功能有明显的抑制作用 。
Objective To investigate the effects of rapamycin on the function of human dendritic cells(DC) Methods DCs were derived from human monocytes upon culture with interleukin (IL) 4 and granulocyte macrophage colony stimulating factor in the presence or absence of rapamycin (0 1-10 ng/mL) Surface costimulatory factor CD86 (7 2) of DC were assessed by flow cytometry Immunostimulatory capacity was measured by mixed lymphocyte reaction (MLR) ELISA was used to analyze the level of cytokines (IL 1β, IL 6, IL 10 and TNF a) in the medium of MLR Results DC differentiated in the presence of rapamycin (RPM DC) had decreased expression of CD86 Allogenic T lymphocyte responses were markedly impaired when RPM DCs were used as stimulator cells Inflammatory cytokines in the medium of MLR were reduced Conclusion Rapamycin has a profound inhibitory effect on DC function, which may be one of its mechanisms in the prevention of restenosis
出处
《中国介入心脏病学杂志》
2004年第4期244-246,共3页
Chinese Journal of Interventional Cardiology
