摘要
目的 :探讨中药“克疣灵”治疗CA的作用机理。方法 :采用细胞原代传代培养技术、MTT比色法测定不同浓度“克疣灵”对兔包皮表皮细胞、CA疣体组织细胞增殖生长的影响 ,采用FQ -PCR定量检测不同浓度“克疣灵”对HPV6 11DNA表达水平及对CA患者阴茎包皮下不同区域HPV6 11DNA表达水平的影响作用。结果 :“克疣灵”对兔包皮表皮细胞增殖无影响 ,而CA疣体组织细胞增殖生长具有显著的抑制作用。结论 :能显著抑制CA疣体组织细胞的增殖生长 ,而对机体正常表皮细胞无损害作用。提示“克疣灵”临床应用安全。
Objective:To discuss the effect mechanism of Traditional Chinese Medicine “Keyouling”in treatment of CA.Methods:Determining the effects of “Keyouling”with different concentrations on growth and proliferation of rabbits' prepuce epithelial cells and CA warts tissue cells by means of primary culture and subculture.Results:“Keyouling” has no effect on proliferation of rabbits' prepuce cuticle cells,but it has significant inhibition on CA warts tissue cells.Concentrations of “Keyouling”assume negative correlation with cell-proliferatiom rate of CA warts tissue cells.Conclusions:“Keyouling”can significantly restrain growth and proliferation of CA warts tissue cells,while it has no damaging effect on normal organic cells.It is cued that“Keyouling”is safe in clinical use.
出处
《皮肤病与性病》
2004年第3期2-4,共3页
Dermatology and Venereology
关键词
克疣灵
尖锐湿疣
细胞增殖
实验
药理作用
Keyouling”
Condyloma acuminatum
Celluar primary culture and subculture Cells proliferation
Laboralory research.
