摘要
目的 :观察异氟醚麻醉后大鼠中枢神经系统c-fos基因的表达 ,了解异氟醚在中枢神经系统的作用部位 ,探讨吸入全麻机理 .方法 :SD大鼠 4 0只 ,雌雄不拘 ,体重 (178 6± 18 0 )g ,日龄 (80± 15 )d ,随机分为对照组、 0 6 %异氟醚组 ,1 2 %异氟醚组和 1 8%异氟醚组 ,每组 10只 .实验组分别吸入 0 6 % ,1 2 %和 1 8%的异氟醚 1h ,对照组除不吸入异氟醚外 ,其余条件完全同实验组 .停止吸入后 ,每只动物腹腔注射 3%戊巴比妥钠 (4 0mg/kg)麻醉后开胸 ,经升主动脉灌注生理盐水 10 0mL洗去全身血液 ,继之灌注多聚甲醛液 (0 1mmol/L ,pH 7 4 ,含 4 %的多聚甲醛 ) 2 5 0mL ,固定 90min .取全脑石蜡包埋 ,切片 (片厚 5 μm) ,应用c-fos基因免疫组织化学方法 ,低倍显微镜 (× 10 )下观察染色阳性细胞的分布情况 ,高倍镜 (× 2 0 )下观察鉴别Fos染色免疫阳性细胞并作阳性细胞记数 .结果 :对照组中未发现Fos阳性细胞 ,实验组大鼠的皮质、丘脑、中脑、桥脑、延髓、小脑可观察到Fos蛋白阳性神经元 ,与对照组相比P <0 0 5和P <0 0 1,这些神经元的分布局限于某些区域 ;同时发现Fos蛋白阳性神经元随吸入异氟醚浓度的增加而增多 ,实验组间相比P <0 0 5和P <0 0 1.结论 :异氟醚能诱导大鼠皮质、丘脑、中脑、桥脑、延髓?
Objectives: To investigate the expression of c-fos oncogene in rats, central nervous system following Isoflurane anesthesia, sdudy Isoflurane action sites in CNS, and discuss general anesthetic mechanisms of inhalation anesthetics. Methods: 40 adult SD rats, weight (178.6±18.0)g, were randomly divided into 4?groups: control group (n=10), 0.6% Isoflurane group (n=10),1.2% Isoflurane group (n=10), 1.8% Isoflurane group (n=10). Experimental groups inhalated 0.6%,1.2%,1.8% Isoflurane for 1?h. The control group underwent the same steps except without inhalating Isoflurane. After 1?h, each rat was anesthetized by intraperitoneal injection pentobarbital (40?mg/kg), then opened, chest and perfused normal saline 100?mL as well 4% poly-formalindehyde 250?mL. 90?min later the whole brain was paraffined-inbeded and sliced. C-fos oncogene immunohistochemical technique was employed to observe and count on the Fos-like immunoreactive (FLI) cells. Results: In control group, there was no FLI cell seen.The experimental groups could see FLI cells in the cortex, thalamus, midbrain, bridgebrain, medulla oblongata and cerebellum. Compared to control group P<0.05 and P<0.01, those FLI cells were limited in some region. Meanwhile finding FLI cells were increased with Isoflurane concentration raised, and among experiment groups the difference P<0.05 and P<0.01. Conclusions: (1) Isoflurane can induce expression of c-fos oncogene in the cortex, thalamus, midbrain, bridgebrain, medulla oblongata and cerebellum of rats .(2) The cortex, thalamus, midbrain, bridgebrain, medulla oblongata, and cerebellum are the action sites of Isoflurane.
出处
《昆明医学院学报》
2004年第3期4-8,共5页
Journal of Kunming Medical College
基金
云南省科技厅资助课题 (2 0 0 0C0 0 6 0M )
