摘要
为了探讨在猪 人异种移植超急性排斥反应中的细胞毒作用及细胞因子白细胞介素 1 (IL 1 )的变化 ,用体外培养的猪血管内皮细胞和人血清共同反应 ,建立猪 人异种移植超急性排斥反应的体外实验模型 ,用乳酸脱氢酶(LDH)释放法观察补体依赖的细胞毒作用 ,换算出内皮细胞溶解率 ,将其作为评定血管内皮细胞激活和损伤的标志 ;采用ELISA法检测上清液中IL 1 β。结果 :1 )在相同时间点上 ,正常人血清组 (HS组 )的细胞溶解率明显高于对照组 ,与其他各组比较无明显差异 ;随着时间的延长 ,HS组内皮细胞溶解率逐渐增高 ,于 3～ 4h接近高峰。 2 )反应 0 .5h后 ,正常人血清组IL 1 β的质量浓度明显高于对照组 (P <0 .0 1 ) ;灭活人血清组、C1q缺乏组与对照组组间比较无显著差异 (P >0 .0 5 ) ;随着时间的延长 ,HS组IL 1 β质量浓度继续升高 (P <0 .0 1 ) ,3～ 4h保持稳定。IL 1的变化过程与内皮细胞溶解率的变化趋势基本一致。结果提示 :在猪 人异种移植中 ,血管内皮细胞激活及IL
The aim was to assess complement dependent cytotoxicity and change of IL-1 in hyperacute rejection of pig-to-human xenotransplantation. Cultural porcine vascular endothelial cells (PVECs) and human sera were used to set up an vitro model of hyperacute rejection of pig-to-human xenotransplantation. Various serums were divided into four groups: HS, HI HS, C1q-D, PS. Every serum was incubated with cultural primary porcine endothelial cells from 0.5 to 4 h, then supernatant fraction was removed to test LDH content, finally percent cytotoxicity was calculated. Morever, IL-1β in supernatant fraction was determined by ELISA. Result: 1) In the same time point, percent cytolysis of HS was higher significantly than that of control (P<0.01) and HI HS, C1q-D’s percent cytolysis was similar to control’s (P>0.05). As time prolonged, percent cytolysis of HS continued to increase ( P< 0.01). However, percent cytolysis of every group didn’t increase after 3～4 h. 2) In the group of HS, the contents of IL-1β were higher than those of control ( P< 0.01). Other groups’ contents were similar to those of control ( P> 0.05). When reaction time prolonged, content in group HS went up and remained stable when reaction time arrived at 3～4 h. The results suggest that endothelial cells have an important role in the thrombosis in hyperacute rejection of pig-to-human xenotransplantation by influencing IL-1.
出处
《首都医科大学学报》
CAS
2004年第3期370-373,共4页
Journal of Capital Medical University
