摘要
为研究接种卡介苗 (BCG)时摇匀疫苗对菌数的影响 ,定量分析BCG摇匀与静置 10min、2 0min、30min时 ,安瓿内上下部分菌数的差异。结果显示 :BCG摇匀时安瓿内上下部分菌数差异无显著的统计学意义 ( 10 6 7± 1 2 3与10 85± 1 12 ;u =1 2 6 ,P >0 0 5 ) ;静置 10min、2 0min、30min后 ,安瓿内上下部分菌数分别是 8 87± 1 36与14 2 8± 1 2 3(u =4 0 1,P <0 0 1)、5 5 8± 1 5 5与 15 6 7± 1 2 7(u =4 39,P <0 0 1)、1 6 9± 1 6 7与 2 0 5 7±1 32 ( u =4 4 4 ,P <0 0 1) ,差异均有非常显著的统计学意义 ;静置与摇匀时的菌数差异亦有非常显著的统计学意义 (P <0 0 1)。因此 ,BCG溶解后 ,必须摇匀使用。摇匀后静置 10min、2 0min、30min时 ,同一安瓿内上下部分的菌数相差 2倍、3倍、12倍 ;与摇匀后比较 ,上部菌数量分别减少至 83 1%、5 2 3%、15 8%。接种未摇匀的BCG 。
To Study the effect of vaccinating evenly shaken BCG on bacteria number,quantitatively analyze the difference of bacteria numbers in the upper and lower parts of the ampoule when shaken evenly,laid down for 10 minutes,20 minutes and 30 minutes respectively The results showed there was no statistical difference between the bacteria numbers in the upper and lower parts of the ampoule when shaken evenly (10 67±1 23 vs 10 85±1 12;U=1 26,P>0 05) There were significant differences between the bacteria numbers in the upper and lower parts of the ampoule which were 8 87±1 36 vs 14 28±1 28,p<0 01,5 58±1 55 vs 15 67±1 27,P<0 01 and 1 69±1 67 vs 20 57±1 32,P<0 01 when laid down for 10 mins,20 mins and 30 mins respectively. There was also significant difference between the bacteria numbers when laid down and evenly shaken (P<0 01) As a result,BCG must be used after dissolved and shaken evenly When laid down for 10,20,30 minutes,the bacteria numbers in the upper were 2,3,12 times more than the lower part of the same ampoule respectively,and they decreased to 83 1%,52 3%,15 8% of the number when shaken evenly respectively It is possible to vaccinate failurey or increase the abverse reaction if injecting unevenly shaken BCG
出处
《中国计划免疫》
2004年第4期238-239,共2页
Chinese Journal of Vaccines and Immunization
关键词
卡介苗
菌数
顶防接种
不良反应
BCG
Bacteria number
vaccination
Adverse reaction
