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假根羽藻(Bryopsis corticulans)中一种管藻黄素-叶绿素a/b蛋白复合体 被引量:1

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摘要 经过连续两步的液相色谱层析分离过程, 从假根羽藻(Bryopsis corticulans)类囊体膜直接分离出来一种捕光蛋白复合体(LHCP). 该捕光蛋白复合体的三聚体经蔗糖密度梯度超速离心分离获得. SDS-PAGE电泳分析结果显示, 这个捕光蛋白复合体至少由5种蛋白质组成, 其中分子量约为31 kD的蛋白质此前尚未在高等植物的主要捕光蛋白复合体(LHCⅡ)中发现. 分离获得的假根羽藻捕光蛋白复合体除了含有叶绿素(Chl) a, Chl b, 新黄素和紫黄素外, 还含有一种特殊的类胡萝卜素─管藻黄素. 假根羽藻管藻黄素-叶绿素a/b捕光蛋白复合体中存在的管藻黄素的作用是加强该色素蛋白复合体对蓝绿区域(530 nm)光的吸收, 假根羽藻LHCP具有与高等植物中的黄体素-叶绿素蛋白复合体相似的吸收谱和荧光发射谱. 管藻黄素和Chl b向Chl a的高效传能说明捕光色素分子高度有序地结合于假根羽藻的捕光蛋白复合体上. 管藻黄素-叶绿素a/b蛋白质使有机体得以增强其吸收在深海或潮下带海域分布较多的蓝绿光.
出处 《科学通报》 EI CAS CSCD 北大核心 2004年第17期1725-1730,共6页 Chinese Science Bulletin
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