摘要
目的 :观察白细胞介素 6 (interleukin 6 ,IL 6 )对血管升压素 (argipressin ,AVP)诱导的心脏成纤维细胞 (CFs)增殖及p2 7蛋白表达的影响。方法 :以培养的新生Sprague Dawley(SD)大鼠CFs为实验模型 ,四氮唑盐 (MTT)比色法检测细胞增殖 ,流式细胞分析仪 (FCM)技术测定细胞周期及p2 7蛋白表达阳性率。结果 :① 10 0 0 0U/mlIL 6作用于CFs 4 8h ,MTT法A值 (0 .38± 0 .0 1)显著高于基础状态组 (0 .32± 0 .0 1,P <0 .0 1) ;10 -7mol/LAVP和 10 0 0 0U/mlIL 6共同作用组CFs的A值 (0 .4 1± 0 .0 1)也明显高于AVP单独作用组 (0 .39± 0 .0 1,P <0 .0 1)。②IL 6作用组CFs的S期细胞百分率 (13.0 %± 3.5 % )和细胞增殖指数 (PI) (2 9.4 %± 1.9% )均高于基础状态组 (7.5 %± 1.0 %和 2 6 .0 %± 1.0 % ,P <0 .0 1) ,G0 /G1期细胞百分率 (70 .6 %± 1.9% )低于基础状态组 (74 .0 %± 1.0 % ) ;AVP +IL 6组CFs的S期细胞百分率和PI分别为 18.8%± 1.5 %和 35 .2 %± 1.6 % ,明显高于AVP组 (15 .5 %± 1.4 %和 31.4 %± 1.5 % ,P<0 .0 1) ,而G0 /G1期细胞百分率 (6 4 .8%± 1.6 % )明显低于AVP组 (6 8.6 %± 1.5 % ,P <0 .0 1)。 (3)IL 6组CFsp2 7蛋白表达阳性率 (72 .6 %± 2 .5 % )明显低于基础状态组 (78.4
AIM: To study the intervention effects of interleu kin-6 (IL-6) on cell proliferation and p27 expression in cardiac fibroblasts (CFs) modulated by argipressin (AVP). METHODS: Neonatal Sprague-Dawley (SD) rats CFs we re cultured as models. MTT assay was used to evaluate cell proliferation. Cell cycle distribution and p27 expression were determined with flow cytometre (FCM). RESULTS : ①A value of CFs measured with MTT assay in the 10 000 U/ml IL-6 group was 0.38±0.01, which was significantly higher than that of basal condition ( 0.3 2±0.01, P<0.01); A value in 10 -7mol/L AVP+10 000 U/ml IL-6 group was 0.41±0.01, which was significantly higher than that of the AVP group ( 0.39±0.01, P<0.01). ②The p ercentage of CFs at stage S (13.0%±3.5%) and proliferation index (PI) (29.4%±1.9%) in the IL-6 group were higher than those of the basal condition (7.5%± 1.0% and 26. 0%±1.0%, P< 0.01), commitment with a significant decrease in the percentage of stage G 0/G 1 cells (70.6%±1.9% vs 74.0%±1.0%, P<0.01); the percentages of CFs at stage S (18.8%±1.5%) and PI (35.2%±1.6%) were much higher in the AVP+IL-6 group than in the AVP group(15.5%±1.4% and 31.4%±1.5%, P<0.01), commitment with a significant decrease in the percentage of stage G 0/G 1 cells (64.8%±1.6% vs 68.6%±1.5%, P<0.01). ③Positive rate of p27 expression in IL-6 group (72.6%±2.5%) was significantly lower than that of basal condition (78.4%±1.9%, P<0.01); Moreover, positive rate of p27 expression in AVP+IL-6 group (60.2%±1.7%) was significantly lower than that of AVP group (63.3%±1.9%, P<0.01). CONCLUSION: IL-6 can enhance CFs proliferation induced by AVP. p27 may be the same target regulatory protein of IL-6 and AVP in CFs.
出处
《心脏杂志》
CAS
2004年第5期397-399,402,共4页
Chinese Heart Journal
基金
国家重点基础研究发展规划 ( 973 )资助 (G2 0 0 0 5 70 0 4)
陕西省科学技术研究发展计划项目资助 [2 0 0 2K10 G1( 2 3 ) ]。
