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核盘菌转代培养后生理特性变异初探 被引量:3

PRELIMINARY STUDIES ON THE PHYSIOLOGICAL CHANGING OF SCLEROTINIA SCLEROTIORUM IN LABORATORY
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摘要 来自向日葵上的核盘菌(Sclerotinia sclerotiorum(Lib.)de·Bary)菌株Sun-1P经多次转代培养及冰箱保存后对有机氮(牛肉汁、蛋白胨、酵母膏)及淀粉的利用能力发生了显著改变。其中转代株Sun-1M对有机氮的利用与pH值及速效糖(葡萄糖)的存在有关系。离体致病性测定表明:Sun-1M对油菜、莴苣、向日葵及蚕豆等寄主仍具有致病性。 After several successive transfers on agar medium and longterm storage at the refigrator, the sunflower strain, Sun-1P. of Sclerotinia sclerotiorum (Lib.) de Bary showed signs of growth decline. It losed the ability of utilizing organic nitrogens such as beef extract, peptone and yeast extract. The new strain Sun-1M derived from Sun-1P could grow on the medium containing the organic nitrogens metioned above suppemented with gluose (2×10^(-2)) or acidified below pH6. 0. Pathogenicity tests in viro on rapeseed (Brassica rapusc, v. E821). lettuce (Lactuca sativa c.v. Yichang Jianye), broadbean (Vicia faba, 78-2) and sunflower (Helianthusannuus) showed that Sun-1M could infect all the crops including Sun-1P.
出处 《华中农业大学学报》 CAS CSCD 北大核心 1993年第1期23-26,共4页 Journal of Huazhong Agricultural University
基金 湖北省自然科学基金
关键词 核盘菌 生理变异 转代培养 Sclerotinia sclerotiorum, physiological changing
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