摘要
来自向日葵上的核盘菌(Sclerotinia sclerotiorum(Lib.)de·Bary)菌株Sun-1P经多次转代培养及冰箱保存后对有机氮(牛肉汁、蛋白胨、酵母膏)及淀粉的利用能力发生了显著改变。其中转代株Sun-1M对有机氮的利用与pH值及速效糖(葡萄糖)的存在有关系。离体致病性测定表明:Sun-1M对油菜、莴苣、向日葵及蚕豆等寄主仍具有致病性。
After several successive transfers on agar medium and longterm storage at the refigrator, the sunflower strain, Sun-1P. of Sclerotinia sclerotiorum (Lib.) de Bary showed signs of growth decline. It losed the ability of utilizing organic nitrogens such as beef extract, peptone and yeast extract. The new strain Sun-1M derived from Sun-1P could grow on the medium containing the organic nitrogens metioned above suppemented with gluose (2×10^(-2)) or acidified below pH6. 0. Pathogenicity tests in viro on rapeseed (Brassica rapusc, v. E821). lettuce (Lactuca sativa c.v. Yichang Jianye), broadbean (Vicia faba, 78-2) and sunflower (Helianthusannuus) showed that Sun-1M could infect all the crops including Sun-1P.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1993年第1期23-26,共4页
Journal of Huazhong Agricultural University
基金
湖北省自然科学基金
关键词
核盘菌
生理变异
转代培养
Sclerotinia sclerotiorum, physiological changing