摘要
本研究依据编码猪细小病毒结构蛋白VP2基因序列,合成了一对寡核苷酸引物,通过减少病毒核酸的提取时间、费用及优化聚合酶链式反应(PCR)的条件,成功地从猪细小病毒(PPV)感染的细胞中扩增出预期的158bp片段,经EcoRⅠ酶切鉴定,证实了该扩增片段的特异性。经敏感性试验测定,PCR的最低检出量为0 008病毒血凝(HA)单位。这些结果表明本试验建立的PCR对PPV的检测人有快速、简便、经济、灵敏度高和特异性强的特点。
A pair of 20_base primers within PPV structure vp2 coding region was synthesized.Through the decrease of time and cost of extracting DNA from PPV_infected PK_15 cells and optimizing PCR conditions,the expected 158?bp fragment was amplified.The amplified fragment was shown to be specific for PPV DNA as indicated by EcoR Ⅰ digestion.These results showed the developed PCR is a fast、simple、economical、specific and sentitive detection method.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2004年第6期465-467,共3页
Chinese Journal of Preventive Veterinary Medicine
