摘要
本研究采用垂直聚丙烯酰胺凝胶电泳技术 ,分析了 3 3头塔里木马鹿的血清白蛋白 (Alb) ,后白蛋白 (Po) ,转铁蛋白 (Tf) ,后转铁蛋白 (Ptf) ,血清蛋白酶抑制物 1 (Pi Ⅰ) ,血清蛋白酶抑制物 2 (Pi Ⅱ) ,后白蛋白 1 (Po Ⅰ) ,后白蛋白 2 (Po Ⅱ)共 8个蛋白座位的基因型频率、基因频率和群体遗传变异指标。结果表明 ,Po Ⅱ和Alb座位呈单态 ,其余各蛋白座位均呈多态。在多态座位中Pi Ⅱ和Po Ⅰ各有 3种基因型 ,分别受两个等位基因所控制。Ptf有 6种基因型 ,受3个复等位基因控制。Po有 4种基因型 ,Tf和Pi Ⅰ各有 3种基因型。群体遗传变异指标 (杂合度 )计算结果表明 ,Ptf,Tf和Po的杂合度较高 ,分别为 0 .6 3 0 5 ,0 .6 1 2 9和 0 .5 94 6 ,有效基因数也较多 ,分别为 2 .70 6 1 ,2 .5 83 6和 2 .4 6 6 6。
Eight blood protein loci (Alb, Po, Tf, Ptf,, Pi-Ⅰ, Pi-Ⅱ, Po-Ⅰ, Po-Ⅱ)of 33 deers in Talimu Wapiti were detected by using vertical polyacrylamide gel electrophoresis. The results showed that Po-Ⅱ and Alb were singlet, Others were polymorphic. The polymorphism of Pi-Ⅱ, Po-Ⅰ, Ptf, Po, Tf and Pi-Ⅰ were exhibited 3, 3, 6, 4, 3 and 3 phenotypes and controlled by 2, 2, 3, 3, 3 and 3 codominant alleles respectively. The heterozygesity of Ptf, Tf and Po were 0.6305, 0.6129 and 0.5946, and the average numbers of alleles per locus were 2.7061, 2.5836 and 2.4666, respectively.
出处
《中国畜牧杂志》
CAS
北大核心
2004年第10期5-9,共5页
Chinese Journal of Animal Science
关键词
畜牧学
血液蛋白多态性
聚丙烯酰胺凝胶电泳
马鹿
Animal science
Blood protein polymorphism
Vertical polyacrylamide gel electrophoresis
Talimu wapiti
