摘要
利用AFLP技术从京玉、美味正反交F1代69株群体中筛选到3个特异扩增片段,与桃果实非酸/酸性状紧密连锁。回收特异片段并将其克隆、测序,结果表明特异片段A、B、C均来自于EcoRI与MseI两内切酶酶切片段,大小依次为140、199、408bp,利用序列分析软件推测片段B、C中可能含有与糖水平调控作用相关的因子。上述结果对AFLP标记向SCAR标记的转换具有重要意义。
Three specific amplified DNA fragments linked to fruit non-acid /acid trait of peach were obtained from 69 F1 seedlings of the intra-specific cross between cultivars 'Jingyu' and 'Flavortop' by using AFLP technique. After cloning the specific fragments and sequencing,we found that fragment A,B and C all were digested by both EcoRⅠand MseⅠ,and were 140 bp,199 bp,and 408 bp in length respectively. It was presumed that fragments B and C might contain elements which are relevant to regulating the sugar content by utilizing sequence analysis software. This study would be important for further SCAR marker conversion.
出处
《果树学报》
CAS
CSCD
北大核心
2004年第6期526-529,共4页
Journal of Fruit Science
基金
北京科技合同项目(954830300)
北京市新星项目(953812200)。
