摘要
利用AFLP分子标记技术对国内外49份扁桃材料的亲缘关系进行了鉴定,使用3对选择性引物组合,每对引物扩增出136条带,并对其进行聚类分析。结果表明,不同种以及不同品种间的遗传距离不同。在相似系数小于0.68时,大多数栽培品种聚为一类,并可将野生扁桃组、苦巴旦组、榆叶梅,以及桃、中国樱桃、欧洲甜樱桃分开。栽培品种中,同一种源区的大多数栽培品种能聚类在一起。我国的扁桃资源与国外的扁桃资源遗传差异较大。
The genetic relationships among 49 almond collections, belonging to 5 species, collected from homeland and abroad were identified via AFLP molecular labeling technique. Three pairs of primers were selected out to amplify 136 polymorphism bands. The genetic distance between these collectiions were analyzed with cluster analysis based on these bands. The result showed that the genetic distance between them were different,and most of the cultivars were classified in the same group when the genetic similarity coefficient was less than 0.68. This group separated cultivars away from Amygdalus ledebouriana, Kubadan, Amygdalus triloba, Cerasus. pseudocerasus and Cerasus avium. The cultivars from the same area were clustered in the same group. There was great genetic discrepancy between the domestic and abroad species.
出处
《果树学报》
CAS
CSCD
北大核心
2004年第6期552-555,共4页
Journal of Fruit Science
基金
国家自然科学基金资助项目(编号30170649)
国家十五攻关课题(编号2001BA707B02)。
