摘要
目的研究增殖细胞核抗原(PCNA)、bcl2及cmyc在甲状腺肿瘤细胞中的表达,并探讨DNA倍体数在甲状腺恶性肿瘤中的诊断价值。方法应用免疫组化技术检测36例甲状腺腺瘤和37例甲状腺腺癌标本PCNA、bcl2及cmyc的表达;并用图像分析技术测定8例甲状腺腺瘤和17例甲状腺腺癌标本的DNA倍体。结果甲状腺腺癌中PCNA阳性率为(43.02±31.16)%,cmyc的阳性表达率为89.2%,均显著高于甲状腺腺瘤(16.15±9.28)%和50.0%(均P<0.05)。而甲状腺腺癌bcl2阳性表达率为64.9%,显著低于甲状腺腺瘤88.9%(P<0.05)。甲状腺腺癌转移的淋巴结中PCNA高于其原发灶,且无bcl2表达。PCNA与bcl2及cmyc在甲状腺肿瘤中无显著相关性。甲状腺腺癌的5倍体细胞百分比显著高于甲状腺腺瘤(P<0.01)。结论bcl2及cmyc可能共同参与甲状腺腺癌的发生发展;PCNA与bcl2的异常表达可能对甲状腺肿瘤的生物学行为及预后的判断有一定参考意义。5倍体细胞大于8%可作为判断甲状腺肿瘤良恶性的参考指标。
Objective To investigate the expressions of proliferating cell nuclear antigen (PCNA), bcl-2, and c-myc protein, and to explore diagnostic value of DNA ploidy in benign and malignant thyroid neoplasms. Methods The expressions of PCNA, bcl-2 and c-myc proteins in 36 cases of thyroid adenomas and 37 cases of thyroid carcinomas were examined by immunohistochemistry technique. DNA ploidy was measured by imaging analysis technique in 8 cases of thyroid adenomas and 17 cases of thyroid carcinomas. Results Among thyroid carcinomas, the positive rates of PCNA (43.02±31.16)% and c-myc protein 89.2% were significantly higher than those of thyroid adenomas (16.15±9.28)% and 50.0% respectively (both P<0.05). Positive rate of bcl-2 in thyroid carcinoma was significantly lower than that of thyorid adenomas 64.9% vs 88.9%, (P<0.05). The positive rate of PCNA in lymph node with metastasis of thyroid carcinoma was higher than that of primitive carcinoma but bcl-2 protein was negative. There were no correlations among PCNA, bcl-2, and c-myc in thyroid neoplasm. The difference in percentages of 5-ploid cells between thyroid adenoma and thyroid carcinoma was statistically significant (P<0.01). Conclusion Both bcl-2 and c-myc proteins may play important roles in the occurrence and development of thyroid carcinoma. PCNA and bcl-2 assessments may be helpful in predicting biological behavior and prognosis of thyroid neoplasm. More than 8% of 5-ploid cells may be a reference index for identifying benign and malignant thyroid neoplasms.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2004年第4期289-292,共4页
Chinese Journal of Endocrinology and Metabolism
基金
贵州省科委资助项目(983055)
