摘要
胰蛋白酶酶解天花粉蛋白,用高效液相色谱分离酶解肽段,用顺序仪测定其有关肽段的顺序。用羧肽酶A,B,Y测定了天花粉蛋白C-端和天花粉蛋白溴化氰降解肽CB1的C-端顺序,修正了我们1985年测定的天花粉蛋白一级结构,证明天花粉蛋白由246(7)氨基酸残基所组成,除C-端微观不均一外,与Collins结果一致。同时比较了芜湖产天花粉蛋白一级结构与平湖产的天花粉蛋白一级结构,没有发现两者的一级结构有差别。
The primary structure of trichosanthin has been revised by sequencing its peptides isolated by reverse-phase HPLO from the tryptic digest of the intact triehosanthin as well as by kinetic studying on the carboxypeptidase (CpA, CpB and CpY) degradation of trichosanthin and of CB-1, the CNBr-degradation fragment of trichosanthin. The revised amino acid sequence consists of 246(7) residues, same with that reported by Collins (1990) except the micro-heterogeneity at its C-terminal. No difference has been shown from the studies of the amino acid sequence of trichosanthin from different places of origin, Wuhu in Anhui province and Pinghu in Zhejiang province.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
1993年第10期1023-1029,共7页
Acta Chimica Sinica
