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嵌合体与同种异体肾移植免疫耐受相关性的研究 被引量:6

Relationship between microchimerism and immunologic tolerance after kidney transplantation
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摘要 目的 探讨同种异体肾移植术后嵌合体表达情况与免疫耐受的相关性。 方法 采用3对引物 ,应用PCR和RT PCR方法 ,检测接受男性供体肾移植的女性受者外周血及尿沉渣Y染色体特异片段DNA和mRNA表达 ,进行嵌合体与免疫耐受相关性研究。 结果  176例女性受者嵌合阳性 137例 ( 77.84 % ) ,阴性 39例 ( 2 2 .16 % )。阳性组平均存活时间 ( 8.9± 3.7)年 ,阴性组 ( 5 .2± 3.9)年 ,差异有显著性意义 (P <0 .0 5 ) ;阳性组发生排斥反应 15例 ( 10 .9% ) ,阴性组 11例 ( 2 8.2 % ) ,差异有显著性意义 (P <0 .0 5 )。 结论 肾移植术后受者体内嵌合状态与免疫耐受相关 。 Objective To investigate the relationship between microchimerism and immunologic tolerance in kidney transplantation recipients. Methods In samples of peripheral blood and urinary sediment from 176 females who received male donor's kidney,DNA and mRNA expression of specific fragment in Y chromosome were determined by PCR and RT PCR. Results The test results were positive for chimerism in 137 out of 176 recipients(77.84%),and negative in 39 cases (22.16%).The mean survival period in chimerism positive group was 8.9±3.7 years,and 5.2±3.9 years in chimerism negative group.The difference was statistically significant between the 2 groups ( P <0.05).Fifteen recipients (10.9%) in chimerism positive group and 11 (28.2%) in chimerism negative group suffered from rejection. The difference between the 2 groups was also significant ( P <0.05). Conclusions Chimerism status of recipients after renal transplantation is related to immunologic tolerance of the body.Microchimerism can be used as a marker in monitoring immunologic tolerance in organ transplantation.
出处 《中华泌尿外科杂志》 CAS CSCD 北大核心 2004年第10期688-690,共3页 Chinese Journal of Urology
基金 吉林省科技厅资助项目 (2 0 0 3 0 5 3 7 1)
关键词 免疫耐受 阴性 嵌合体 阳性 同种异体肾移植 受者 相关性 结论 生物学 显著性 Kidney transplantation Chimra Immune tolerance
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  • 1Starzl TE, Demetris AJ, Murase N, et al. Cell migration, chimerism,and graft acceptancet. Lancet, 1992,339:1579-1582.
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  • 6陈惠萍,曾彩虹,季曙明,尹广,陈劲松,朱茂艳,黎磊石.肾组织活检在移植肾功能损害的诊断与鉴别诊断中的价值[J].中华器官移植杂志,2001,22(1):5-7. 被引量:10
  • 7史其新,谢蜀生,俞莉章,郭应禄.成年大鼠联体诱导的移植耐受与嵌合状态相关性的研究[J].中华医学杂志,2000,80(8):602-605. 被引量:6
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