摘要
目的 检测非霍奇金淋巴瘤 (NHL)抗原受体基因双重重排的发生率及初步探讨其病理意义和可能机制。方法 采用PCR方法 ,联合使用IgHFR3A及FR2A、TCRγ、TCRβ引物 ,检测 12 5例NHL患者IgH基因及TCR基因克隆性重排 ;检测B NHL和T NHL中抗原受体基因双重重排的发生率 ,并对其中的 117例NHL患者采用免疫组织化学EnVision两步法检测Ki6 7蛋白表达并计算增殖指数 ,分析NHL不同重排类型与增殖指数间的关系。结果 联合使用IgHFR3A及FR2A、TCRγ、TCRβ引物 ,96例B NHL患者中有 8例 (8% )检测到发生双重重排 ,2 9例T NHL患者中有 5例 (17% )检测到发生双重重排。B NHL中 6 5例检测到IgH基因克隆性重排 ,8例发生双重重排 ,15例为多克隆重排 ;T NHL中有 15例检测到TCR基因克隆性重排 ,5例发生双重重排 ,9例为多克隆重排。上述病例同时平行检测了Ki6 7,并计算增殖指数 ,经One way检验 ,在B NHL和T NHL中 ,克隆性重排、双重重排及多克隆重排的淋巴瘤增殖指数差异无显著性 (P >0 .0 5 )。结论 淋巴瘤抗原受体基因的双重重排在成熟NHL中并非罕见 ;
Objective To investigate the rate of dual rearrangements of lymphocytic antigen receptor genes in non-Hodgkin lymphomas (NHL) and its pathogenesis and pathologic significance. Methods PCR analysis of monoclonal, polyclonal and dual rearrangements of IgH and TCRγ, TCRβ genes was carried out in 125 cases of NHL to evaluate the rate of dual rearrangements, immunohistochemistry was performed for a Ki67 protein expression in 117 cases and the proliferation index was calculated. The relationship between antigen receptor gene rearrangements and proliferation index was analyzed. Results Combination of the two pairs of IgH gene primers with the multiplex PCR for TCRγ and TCRβ gene revealed dual rearrangements in 8% (8/96)of B-NHL, 17% (5/29)of T-NHL. In B cell NHL, IgH gene monoclonal, dural and polyclonal rearrangements were identified in 65, 8 and 15 cases respectively, while in T-cell NHL, they were in 15, 5 and 9 cases, respectively. There was no significant difference between proliferation index and monoclonal, dual, polyclonal rearrangements in both B-NHL and T-NHL by One-way test. Conclusion Dual rearrangements in NHL are not rare and have no relationship with proliferation index.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2004年第10期583-587,共5页
Chinese Journal of Hematology
基金
浙江省科技厅科研基金资助项目 ( 0 0 1110 42 5 )
浙江省卫生厅科研基金资助项目 ( 2 0 0 2ZX0 10
2 0 0 3A0 2 0 )
