摘要
目的:探讨大蒜素对海马各区及额、颞叶皮质神经细胞的保护作用及其对血管性痴呆的干预疗效。方法:健康清洁级老龄Wistar大鼠110只分为Pulsinelli四血管阻断(4VO)组、大蒜素治疗组及对照组。应用穿梭箱系统检测大鼠的学习记忆能力,采用甲苯胺蓝染色法计数海马各区及额、颞叶皮质神经细胞。结果:4VO组大鼠的主动回避反应(activeavoidanceresponse,AAR)比率在术后明显下降(P<0.01),大蒜素治疗组大鼠在手术1周后AAR比率为(76.00±8.70)%,明显高于4VO组(69.67±6.94)%,差异有显著性意义(P<0.05)。大蒜素治疗组术后3dCA1区及CA3区神经细胞丧失率(lossrate,LR)明显小于4VO组(P<0.05),在术后1周以后与4VO组比较差异有非常显著性意义(P<0.01)。大蒜素治疗组额叶皮质神经细胞LR在术后3d以后低于4VO组(P<0.05)。齿状回及颞叶皮质神经细胞LR在术后1周以后低于4VO组(P<0.05)。结论:大蒜素可减少实验大鼠海马各区及额、颞叶皮质神经细胞的丢失,可改善实验大鼠的学习记忆能力,可能具有预防和治疗血管性痴呆的作用。
AIM: To probe into the protective effect of garlicin on the neurons of hippocampus and cortex of temporal and frontal lobes, and its curative effect on the intervention of vascular dementia(VD). METHODS: A total of 110 healthy clean class aging Wistar rats were divided into Pulsinelli four-vessel occlusion (4VO) group, garlicin treatment group and control group. The shuttle-box system was used to test the learning and memory abilities of the rats, and toluidine blue stained was used to account the remained neural cells in the hippocampus and cortex of temporal and frontal lobes. RESULTS: The active avoidance response(AAR) ratio after treatment in the 4VO group was obviously decreased(P< 0.01). The AAR ration 1 week after operation in the garlicin treatment group [(76.00±8.70)%] was significantly higher than that in the control group [(69.67±6.94)%](P< 0.05). The loss rate (LR) of nerve cells in the CA1 and CA3 areas in the garlicin treatment group 3 days after operation was obviously less than that in the 4VO group (P< 0.05), and very significantly different from that in the 4VO group 1 week after operation(P< 0.01). The LR of neural cells in the cortex of frontal lobe 3 days after operation was lower than that in the 4VO group(P< 0.05).The LR of neural cells in dentate gyrus and cortex of temporal lobe 1 week after operation in the garlicin treatment group was lower than that in the 4VO group (P< 0.05). CONCLUSION: Garlicin can decrease the loss of neural cells in the hippocampus and cortex of temporal and frontal lobes and improve the learning and memory abilities of the rats as well. Garlicin may have the effects to protect and treat vascular dementia.
出处
《中国临床康复》
CSCD
2004年第31期6934-6935,共2页
Chinese Journal of Clinical Rehabilitation
基金
重庆市科委面上项目(5657)~~
