摘要
目的:从细胞分子水平研究刺五加皂甙(acanthopanaxsenticosussaponins,ASS)对培养神经元缺氧复氧损伤的保护作用及其机制。方法:取孕13～15dICR胎鼠大脑皮质神经元进行原代分离培养,建立缺氧复氧诱导的皮质神经元损伤模型。随机分成正常对照组、缺氧复氧组及ASS组;用MTT法测定神经元存活率,用硝酸还原酶法测定细胞培养上清液中一氧化氮合酶(NOS)的含量,用流式细胞仪检测神经元凋亡率。结果:神经元缺氧2,4,6,8h复氧24h后,存活率分别为(0.604±0.022)%,(0.592±0.017)%,(0.543±0.037)%,(0.534±0.021)%;缺氧8h复氧24h后,神经元凋亡率由(4.13±0.87)%增加至(31.34±0.85)%,NOS含量由(5.23±0.28)μmoL/L增加至(11.39±0.21)μmoL/L(P<0.01);ASS组神经元存活率、神经元凋亡率、NOS含量分别为(0.636±0.021),(16.37±0.66)%,(8.02±0.18)μmoL/L,与缺氧8h复氧24h比较差异有非常显著性意义(P<0.01)。结论:ASS对缺氧复氧引起的神经元损伤有保护作用;ASS可能是通过抑制一氧化氮的释放、抑制神经元凋亡来拮抗神经元损伤。
AIM:To study the effect and mechanism of acanthopanax senticosus saponins (ASS) in protecting cortical neuron injury induced by anoxia/reoxygenation. METHODS:Cortical neurons were removed from 13 to 15-day-old embryonic ICR mice fetuses for primary cultivation,the model of cortical neuron damage were induced by anoxia/reoxygenation.All the rats were divided into normal control group,anoxia/reoxygenation group and ASS group.The neuron survival rate was measured by MTT assay,nitric oxide synthase(NOS) contents in the supernate of culture media were determined by nitrate reductase assay and the apoptosis rate of neuron was measured by flow cytometer. RESULTS:The survival rates of the reurons at anoxia 2,4,6,8 hours and reoxygenation for 24 hours were(0.604±0.022)%,(0.592±0.017)%,(0.543±0.037)%and(0.534±0.021)%respectively;Anoxia 8 hours reoxygenation for 24 hours,the apoptosis rate of neurons was increased from (4.13±0.87)%to(31.34±0.85)%,the NOS content was increased from (5.23±0.28) to(11.39±0.21) μmol/L (P< 0.01).The survival rate, apoptosis rate and NOS content in the ASS group were(0.636±0.021)%, (16.37±0.66)%and(8.02±0.18)μmol/Lrespectively,significantly different from those at anoxia 8 hours reoxygenation for 24 hours(P< 0.01). CONCLUSION:ASS plays a protective role in the cortical neuron damage induced by anoxia-reoxygenation via inhibiting the release of NO and neuron apoptosis.
出处
《中国临床康复》
CSCD
2004年第31期6964-6965,共2页
Chinese Journal of Clinical Rehabilitation
基金
江苏省社会科技发展基金项目(BS2000040)
